{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:55:40Z","timestamp":1781585740613,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LH2021H046"],"award-info":[{"award-number":["LH2021H046"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n The present study aimed to investigate the expression and biological behaviour of the six\u2010transmembrane epithelial antigen of the prostate 1 (STEAP1) in oral squamous cell carcinoma (OSCC), and to further analyse its underlying mechanisms.<\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n Western blot and immunohistochemistry were used to detect protein expression. After overexpression of STEAP1 in OSCC cells by plasmid transfection, cell proliferation, migration, and invasion abilities were assessed using CCK\u20108, scratch, and Transwell assays, respectively, and ROS levels were detected using the corresponding kits. Finally, the expression changes of EMT and Wnt\/\u03b2\u2010catenin pathway\u2010related proteins were analysed by Western blot.<\/jats:p>\n <\/jats:sec>\n \n Result<\/jats:title>\n \n Western blot (WB) and immunohistochemical (IHC) analyses showed that STEAP1 was significantly underexpressed (\n p<\/jats:italic>\n \u2009<\u20090.0001) in oral squamous cell carcinoma (OSCC) tissues and cell lines (SAS, Tca\u20108113, SCC15) compared to normal controls. Functional experiments showed that overexpression of STEAP1 effectively inhibited the proliferation (\n p<\/jats:italic>\n \u2009<\u20090.001), migration (\n p<\/jats:italic>\n \u2009<\u20090.0001), and invasion (\n p<\/jats:italic>\n \u2009<\u20090.001) abilities of OSCC cells (SAS, Tca\u20108113) and reduced intracellular ROS levels (\n p<\/jats:italic>\n \u2009<\u20090.0001). Molecularly, STEAP1 overexpression up\u2010regulated E\u2010cadherin (\n p<\/jats:italic>\n \u2009<\u20090.01) and down\u2010regulated N\u2010cadherin (\n p<\/jats:italic>\n \u2009<\u20090.05), inhibiting the EMT process; meanwhile, it decreased the protein levels of \u03b2\u2010catenin (\n p<\/jats:italic>\n \u2009<\u20090.05), Axin2 (\n p<\/jats:italic>\n \u2009<\u20090.05), and c\u2010Myc (\n p<\/jats:italic>\n \u2009<\u20090.05), as well as the protein levels of the p\u2010 GSK3\u03b2\/T\u2010GSK3\u03b2 ratio (\n p<\/jats:italic>\n \u2009<\u20090.05), but there was no significant change in total GSK3\u03b2 protein, suggesting inhibition of the Wnt\/\u03b2\u2010catenin pathway.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n STEAP1 is downregulated in OSCC and suppresses malignant phenotypes of OSCC cells in\u00a0vitro, with effects associated with EMT\u2010related changes and attenuation of Wnt\/\u03b2\u2010catenin\u2010associated signalling.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/cam4.71862","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:42:34Z","timestamp":1777351354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["STEAP1<\/scp>\n Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt\/\u03b2\u2010Catenin Signalling and\n EMT<\/scp>"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1453-0643","authenticated-orcid":false,"given":"Kuangyu","family":"Jin","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shitong","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jin","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runze","family":"Lu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, School of Stomatology Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.70076"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.6282"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1485174"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jormas.2020.12.003"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.71.1176"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.22941.1"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14164034"},{"key":"e_1_2_12_10_1","doi-asserted-by":"crossref","unstructured":"R. S.Hubert I. V.Vivanco E.Chen et\u00a0al. a prostate\u2010specific cell\u2010surface antigen highly expressed in human prostate tumors. Proceedings of the National Academy of Sciences of the United States of America 1999.","DOI":"10.1073\/pnas.96.25.14523"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.013690"},{"issue":"5","key":"e_1_2_12_12_1","first-page":"812","article-title":"AMG 509 (Xaluritamig), an Anti\u2010STEAP1 XmAb 2 + 1 T\u2010Cell Redirecting Immune Therapy With Avidity\u2010Dependent Activity Against Prostate Cancer. Cancer","volume":"14","author":"Nolan\u2010Stevaux O. L. C.","year":"2024","journal-title":"Discovery"},{"issue":"1","key":"e_1_2_12_13_1","article-title":"Targeting Advanced Prostate Cancer With STEAP1 Chimeric Antigen Receptor T Cell and Tumor\u2010Localized IL\u201012 Immunotherapy. 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