{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:35:30Z","timestamp":1791995730741,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82472629"],"award-info":[{"award-number":["82472629"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Molecular testing has emerged as a pivotal tool for the preoperative assessment of cytologically indeterminate thyroid nodules. In this cross\u2010sectional study, we evaluated the diagnostic utility of a targeted next\u2010generation sequencing (NGS) 4\u2010gene panel, including\n                    <jats:italic>BRAF<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>V600E<\/jats:italic>\n                    <\/jats:sup>\n                    ,\n                    <jats:italic>TERT<\/jats:italic>\n                    promoter mutations,\n                    <jats:italic>RET<\/jats:italic>\n                    fusions, and\n                    <jats:italic>NTRK3<\/jats:italic>\n                    fusion, for enhancing the cytological diagnosis of thyroid nodules prior to surgical intervention. A total of 827 thyroid nodules subjected to fine\u2010needle aspiration and subsequent histopathological confirmation were analyzed, among which 773 (93.5%) were classified as malignant or noninvasive follicular thyroid neoplasm with papillary\u2010like nuclear features (NIFTP). The observed prevalence of molecular alterations was:\n                    <jats:italic>BRAF<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>V600E<\/jats:italic>\n                    <\/jats:sup>\n                    , 68.3% (526\/770);\n                    <jats:italic>TERT<\/jats:italic>\n                    promoter mutations, 10.3% (79\/770);\n                    <jats:italic>RET<\/jats:italic>\n                    fusions, 10.3% (79\/770); and\n                    <jats:italic>NTRK3<\/jats:italic>\n                    fusion, 3.9% (30\/770). Notably, the 4\u2010gene NGS panel demonstrated brilliant diagnostic performance for indeterminate cytological nodules (Bethesda categories III\u2013V), achieving a sensitivity of 87.9%, specificity of 96.3%, positive predictive value (PPV) of 99.7%, negative predictive value (NPV) of 35.9%, and overall accuracy of 88.2%. These findings indicate that the targeted NGS 4\u2010gene panel provides high diagnostic precision in distinguishing benign from malignant nodules. Its implementation offers a cost\u2010effective, efficient molecular diagnostic strategy that may reduce unnecessary diagnostic procedures and facilitate optimized clinical management.\n                  <\/jats:p>","DOI":"10.1002\/cam4.71924","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T05:34:51Z","timestamp":1777959291000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Utility of a Cost\u2010Effective Four\u2010Gene Next Generation Sequencing Panel for Predicting Papillary Thyroid Carcinoma in Indeterminate Thyroid Cytology: A Multicenter Study in China"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-4019","authenticated-orcid":false,"given":"Qiong","family":"Jiao","sequence":"first","affiliation":[{"name":"Department of Pathology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Molecule and Pathology Affiliated Cancer Hospital of Zhengzhou University &amp; Henan Cancer Hospital  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4661-3681","authenticated-orcid":false,"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital of Soochow University Changzhou First People's Hospital  Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3754-9457","authenticated-orcid":false,"given":"Xuling","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pathology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai RIGEN Biotechnology co., Ltd.  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