{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:53:35Z","timestamp":1781585615462,"version":"3.54.5"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872937"],"award-info":[{"award-number":["81872937"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073934"],"award-info":[{"award-number":["82073934"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cancer\u2010associated fibroblasts (CAFs) in the bone microenvironment play a key role in breast cancer (BC) osteolytic bone metastases. Differentiated embryo\u2010chondrocyte expressed gene 1 (DEC1) has been implicated as a potential therapeutic target in CAFs. Here, we investigate the role of DEC1 within the bone microenvironment during BC cell\u2010induced bone destruction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In vivo, a BC bone metastasis mouse model by intratibially injecting 4\u2009T1 cells into DEC1\n                      <jats:sup>+\/+<\/jats:sup>\n                      and DEC1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice was used to explore the effects of DEC1 deficiency in the bone microenvironment on BC osteolytic bone destruction. Bone mesenchymal stromal cells (BMSCs) isolated from DEC1\n                      <jats:sup>+\/+<\/jats:sup>\n                      and DEC1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice were induced to differentiate into CAFs, as in\u00a0vitro model to explore the roles and underlying mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DEC1 deficiency markedly attenuated BC\u2010induced osteolytic destruction. Notably, DEC1\n                      <jats:sup>+\/+<\/jats:sup>\n                      \u20104\u2009T1 mice exhibited a higher abundance of CAF biomarker\u2010positive cells in the bone microenvironment compared to DEC1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      \u20104\u2009T1 mice. Mechanistically, tumor\u2010conditioned medium (TCM) and IL\u20106 were found to promote the differentiation of BMSCs into CAFs through activation of the JAK2\/STAT3 pathway, whereas DEC1 deletion suppressed this process by inhibiting JAK2\/STAT3 signaling. Furthermore, DEC1 deficiency reduced RANKL secretion, thereby limiting osteoclastogenesis, and decreased PAI\u20101 levels, which contributed to remodeling of the tumor microenvironment and suppression of BC cell migration.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings demonstrate that DEC1 deficiency in the bone microenvironment critically restrains BC cell\u2010induced bone destruction through inhibition of CAF differentiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.71940","type":"journal-article","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:21:02Z","timestamp":1778908862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>DEC1<\/scp>\n                    Deficiency Attenuates Breast Cancer\u2010Induced Osteolytic 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