{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T03:29:17Z","timestamp":1786418957739,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy, Government of India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The processes regulating cellular dormancy in tumor cells remain inadequately characterized. Further, there are very few models available that can recapitulate a dormancy\u2010like phenotype in\u00a0vitro. In this study, we investigated the role of microenvironmental cues in inducing a dormancy\u2010like phenotype in estrogen receptor\u2010positive (MCF\u20107) and triple\u2010negative (MDA\u2010MB\u2010231) breast cancer cell lines. We characterized an in\u00a0vitro model in distinct conditions defined hierarchically by the following: (a) normoxia or true hypoxia, (b) dishes coated with fibronectin or laminin or none, and (c) presence or absence of 10\u2009ng\/mL basic fibroblast growth factor (FGF\u20102) in the culture medium, added on day 0 of culture. Cells were cultured at clonogenic densities for 9\u2009days (day \u22121, day 0, up to day +7). Cells were characterized for dormancy\u2010 like behavior using increased p\u2010p38 and decreased p\u2010ERK expression (dormant, p\u2010p38\n                    <jats:sub>High<\/jats:sub>\n                    and p\u2010ERK\n                    <jats:sub>Low<\/jats:sub>\n                    expression), reduced Ki67 expression, elevated p21 and p27 levels by immunofluorescence, absence of senescence using \u03b2\u2010galactosidase staining and resistance to doxorubicin. Fibronectin or laminin were sufficient for the induction of a dormancy\u2010like phenotype in MCF\u20107 cells under hypoxic conditions, whereas the addition of FGF\u20102 to fibronectin (but not laminin) could induce a dormancy\u2010like phenotype under normoxic conditions. FGF\u20102 was required with laminin to induce dormancy in MDA\u2010MB\u2010231 cells under hypoxic conditions (FGF\u20102 plus fibronectin were unable to induce dormancy), whereas laminin or fibronectin alone were sufficient under normoxic conditions. Overall, we established culture conditions that recapitulate an in\u00a0vitro breast cancer dormancy\u2010like phenotype and reveal subtype\u2010specific differences in dormancy regulation.\n                  <\/jats:p>","DOI":"10.1002\/cam4.72059","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T03:42:27Z","timestamp":1782704547000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microenvironmental Cues That Regulate Cancer Cell Dormancy in Estrogen\u2010Receptor Positive and Triple\u2010Negative Breast Cancer Cell Lines"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6071-2278","authenticated-orcid":false,"given":"Jinesh","family":"Maniar","sequence":"first","affiliation":[{"name":"Clinician Scientist Lab, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre  Navi Mumbai 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