{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T03:29:25Z","timestamp":1786418965218,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 114\u20102314\u2010B\u2010038\u2010050\u2010MY3"],"award-info":[{"award-number":["NSTC 114\u20102314\u2010B\u2010038\u2010050\u2010MY3"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020950","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 114\u20102320\u2010B\u2010038\u2010041\u2010MY3"],"award-info":[{"award-number":["NSTC 114\u20102320\u2010B\u2010038\u2010041\u2010MY3"]}],"id":[{"id":"10.13039\/501100020950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009974","name":"Cardinal Tien Hospital","doi-asserted-by":"publisher","award":["CTH114A\u20102003"],"award-info":[{"award-number":["CTH114A\u20102003"]}],"id":[{"id":"10.13039\/501100009974","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Triple\u2010negative breast cancer (TNBC) is a subtype of breast cancer that lacks the expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor\u20102 (HER2). There is a lack of predictive biomarkers for the response of patients with TNBC to targeted therapies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatics analysis was conducted to generate differentially expressed genes (DEGs) of the mammalian target of rapamycin complex 1 (MTORC1) gene set between normal tissues and primary tumors derived from TNBC patients using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Cox regression analysis was performed to identify independent prognostic factors. Endogenous expression levels of the identified prognostic genes were detected in a panel of TNBC cell lines and breast cancer tissues using western blotting and immunohistochemistry (IHC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our findings revealed two prognostic genes: FADS2 and TOMM40. TOMM40 (HR\u2009=\u20092.243) was a risk factor and FADS2 was a protective factor (HR\u2009=\u20090.652). A higher TOMM40\/FADS2 ratio is associated with poor outcomes in patients with TNBC. The TOMM40\/FADS2 ratio was significantly (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) associated with age, tumor size, lymph node metastasis, pathologic stage, and overall survival of patients with TNBC. Remarkably, the MTT cytotoxicity assay revealed that TNBC cells, which possess a higher TOMM40\/FADS2 ratio than TNBC cells with a lower TOMM40\/FADS2 ratio, are more sensitive to mammalian target of rapamycin (mTOR) inhibitor treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results provide a new therapeutic strategy using the TOMM40\/FADS2 expression ratio to predict the cellular sensitivity to mTOR inhibitor treatment in TNBC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.72089","type":"journal-article","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T11:45:56Z","timestamp":1784375156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>TOMM40<\/scp>\n                    \/\n                    <scp>FADS2<\/scp>\n                    Expression Ratio Predicts the Sensitivity to\n                    <scp>mTOR<\/scp>\n                    Inhibitors in Triple\u2010Negative Breast Cancer"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2386-2223","authenticated-orcid":false,"given":"Salman Mohamed","family":"Farah","sequence":"first","affiliation":[{"name":"International Ph.D. Program in Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010Quan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Division of Pulmonary Medicine, Department of Internal Medicine, Shuang ho Hospital Taipei Medical University  New Taipei City Taiwan"},{"name":"Division of Pulmonary Medicine, Department of Internal Medicine, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon Tsung\u2010Ju","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Dermatology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Dermatology Taipei Medical University Hospital  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao\u2010Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Otolaryngology Head and Neck Surgery, Shuang ho Hospital Taipei Medical University  New Taipei City Taiwan"},{"name":"Department of Otolaryngology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"Department of Surgery Taipei Medical University Hospital, Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Hao","family":"Kuei","sequence":"additional","affiliation":[{"name":"School of Medicine Fu\u2010Jen Catholic University  New Taipei City Taiwan"},{"name":"Division of Urology, Department of Surgery, Cardinal Tien Hospital Fu\u2010Jen Catholic University  New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Yu","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Medicine Fu\u2010Jen Catholic University  New Taipei City Taiwan"},{"name":"Division of Breast Surgery and General Surgery, Department 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