{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T23:36:51Z","timestamp":1789688211424,"version":"4.0.1"},"reference-count":60,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sialic acid\u2010binding immunoglobulin\u2010like lectin 10 (SIGLEC10), a member of the SIGLEC family, is selectively expressed on multiple immune cell subsets and plays a key role in immune regulation through the recognition of sialylated ligands. Recent studies suggest that SIGLEC10 functions as an emerging immune checkpoint that contributes to the regulation of tumor\u2010associated macrophages (TAMs) within the tumor microenvironment (TME).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Immunohistochemistry was performed to assess SIGLEC10 expression in a CRC cohort comprising 202 patients, and the association between SIGLEC10 expression and patient survival and prognosis was evaluated. Immunofluorescence staining and flow cytometry were used to characterize the localization and phenotypic features of SIGLEC10\u2010expressing cells within tumor tissues. The Cancer Genome Atlas (TCGA) and RNA sequencing (RNA\u2010seq) datasets were analyzed to investigate SIGLEC10\u2010associated tumor immune signaling pathways. Additionally, single\u2010cell datasets from Tumor Immune Single\u2010cell Hub 2 (TISCH2) were used to further investigate the regulatory mechanisms of SIGLEC10\n                      <jats:sup>high<\/jats:sup>\n                      TAMs within the TME. Finally, the therapeutic potential of SIGLEC10 as an immunotherapeutic target in CRC was evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      High SIGLEC10 expression predicts poor prognosis in CRC patients and serves as an independent prognostic factor. SIGLEC10 is predominantly expressed in CD68\n                      <jats:sup>+<\/jats:sup>\n                      macrophages and is associated with TAMs phenotypes. Functional enrichment analyses indicate that SIGLEC10\n                      <jats:sup>high<\/jats:sup>\n                      TAMs are involved in the regulation of multiple inflammatory and tumor immune signaling pathways. Immune infiltration and differential analyses suggest that SIGLEC10\n                      <jats:sup>high<\/jats:sup>\n                      TAMs may facilitate tumor immune evasion by promoting increased infiltration of immune cells and recruiting various immune chemokines. Single\u2010cell analyses reveal that SIGLEC10\n                      <jats:sup>high<\/jats:sup>\n                      TAMs may remodel the TME through TNFSF13B\u2010mediated regulation of multiple immune cell populations, including B cells. Finally, SIGLEC10 exhibits considerable potential as an immunotherapeutic target for CRC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study highlights SIGLEC10 as an independent prognostic factor in patients with CRC and its potential as a promising target for immunotherapy in CRC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.72106","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T04:19:47Z","timestamp":1784780387000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High SIGLEC10\u2010Expressing Tumor\u2010Associated Macrophages Participate in Remodeling the Tumor Immune Microenvironment and Predict Poor Prognosis in Colorectal Cancer"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3055-8752","authenticated-orcid":false,"given":"Chuang","family":"Miao","sequence":"first","affiliation":[{"name":"Department of General Surgery, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong 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