{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:39:05Z","timestamp":1792809545820,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Phagocytosis checkpoints, such as CD47, have emerged as promising targets for cancer immunotherapy and are being investigated in clinical trials. Despite encouraging findings, reliable biomarkers for monitoring immunotherapeutic responses in triple\u2010negative breast cancer (TNBC) remain tracking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this project is to characterize the functional states of tumor\u2010associated macrophages (TAMs) using macrophage\u2010related imaging probes and assess their potential for monitoring responses to anti\u2010CD47 immunotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Anatomical imaging visualized tumor nodule formation and angiogenesis in tumor\u2010bearing mice. Conventional fluorescence imaging demonstrated the TNBC\u2010targeting ability of monocyte chemoattractant protein\u20101 (MCP\u20101) fluorescein isothiocyanate (FITC) and Dextran\u2010Alexa Fluor 680 (AF 680). Intravital imaging elucidated the pharmacokinetics of the imaging probes in TAMs following intravenous injection. These probes preferentially targeted macrophages, as confirmed by the colocalization between F4\/80\u2010, CD86\u2010, and CD206\u2010binding cells and imaging probe\u2010targeted cells. Tumor\u2010bearing mice that received intravenous injections\u00a0of the imaging probes were sacrificed, and the TNBC tissue was cryo\u2010sectioned, stained, and imaged on a confocal microscope, showing a high molecular targeting ability of the imaging probes for macrophages. The M1\/M2 macrophage ratio was quantified for monitoring the response to anti\u2010CD47 immunotherapy, which demonstrated that MCP\u20101 and Dextran are useful tools for M1 and M2 macrophage identification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study documents a molecularly targeted construct for TAMs in\u00a0vivo and verifies proof of the utility of using the M1\/M2 ratio as a strategy for monitoring the response to CD47 treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.72232","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:09:04Z","timestamp":1791522544000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Immunoscoring Technique to Monitor Anti\u2010\n                    <scp>CD47<\/scp>\n                    Immunotherapy Efficacy in Triple\u2010Negative Breast Cancer"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8277-8139","authenticated-orcid":false,"given":"Minfeng","family":"Yang","sequence":"first","affiliation":[{"name":"School of Public Health Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5117-7442","authenticated-orcid":false,"given":"Shiqiang","family":"Hou","sequence":"additional","affiliation":[{"name":"The Affiliated Chuzhou Hospital of Anhui Medical University, The First People's Hospital of Chuzhou  Chuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0025-7135","authenticated-orcid":false,"given":"Chi Ming","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Health Technology and Informatics The Hong Kong Polytechnic University  Kowloon Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Health Technology and Informatics The Hong Kong Polytechnic University  Kowloon Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5142-9024","authenticated-orcid":false,"given":"Chunjin","family":"Jin","sequence":"additional","affiliation":[{"name":"The Affiliated Chuzhou Hospital of Anhui Medical University, The First People's Hospital of Chuzhou  Chuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Bai","family":"Liu","sequence":"additional","affiliation":[{"name":"Suzhou Key Laboratory of Medical Biotechnology Suzhou Vocational Health College  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8865-0424","authenticated-orcid":false,"given":"Jung Sun","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Health Technology and Informatics The Hong Kong Polytechnic University  Kowloon Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/curroncol29050247"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010019\u20100183\u2010z"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153550"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1166487"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102022\u2010005199"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312197110"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c01210"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2016.1276135"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13267"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc200405d"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102172\u201014\u20106"},{"key":"e_1_2_13_13_1","first-page":"247","volume-title":"Methods in Molecular Biology","author":"Sckell A.","year":"2016"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201006\u20101823"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737140.2025.2610263"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2025.102924"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1362641"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e28385"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2025.103023"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201071746\u2010z"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101456\u20100"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd6167"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1391973"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2019.10618"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16547"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121623109"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.72232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.72232","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.72232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:09:21Z","timestamp":1792807761000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.72232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/cam4.72232"],"URL":"https:\/\/doi.org\/10.1002\/cam4.72232","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2025-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e72232"}}