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Although the impact of specific genetic alterations on drug sensitivity has been reported in BCP\u2010ALL, the effects of\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations on sensitivity to individual agents remain poorly defined in the clinical setting because of the use of combination chemotherapy regimens. In the present study, we addressed this issue through two complementary analyses using a large panel of BCP\u2010ALL cell lines. Initially, 30 cell lines harboring\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations demonstrated significantly greater resistance to daunorubicin (DNR), mafosfamide (MAF; an active analog of cyclophosphamide), 6\u2010mercaptopurine (6\u2010MP), and prednisolone compared to 46 cell lines without mutations. Second, 11 CRISPR\/Cas9\u2010mediated\n                    <jats:italic>TP53<\/jats:italic>\n                    \u2010knockout sublines derived from cell lines with wild\u2010type\n                    <jats:italic>TP53<\/jats:italic>\n                    recapitulated resistance to DNR, MAF, and 6\u2010MP, as well as to vincristine (VCR) and cytarabine (Ara\u2010C). Consistent with these findings, re\u2010evaluation of genome\u2010wide CRISPR screening datasets revealed significant enrichment of\n                    <jats:italic>TP53<\/jats:italic>\n                    gRNAs in cells treated with DNR, MAF, 6\u2010MP, Ara\u2010C, and VCR. Furthermore, transcriptomic and proteomic analyses of p53 target genes in the two cell line models, along with re\u2010evaluation of genome\u2010wide CRISPR screening datasets, identified\n                    <jats:italic>BAX<\/jats:italic>\n                    ,\n                    <jats:italic>BBC3<\/jats:italic>\n                    , and\n                    <jats:italic>FDXR<\/jats:italic>\n                    as key p53 target genes associated with multidrug resistance. These results highlight the potential value of targeting these p53 downstream pathways to improve outcomes in\n                    <jats:italic>TP53<\/jats:italic>\n                    \u2010mutated BCP\u2010ALL cases.\n                  <\/jats:p>","DOI":"10.1002\/cam4.72386","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:13:36Z","timestamp":1791951216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of p53 Target Genes on Drug Resistance in\n                    <scp>\n                      <i>TP53<\/i>\n                    <\/scp>\n                    \u2010Mutated B\u2010Cell Precursor Acute Lymphoblastic Leukemia"],"prefix":"10.1002","volume":"15","author":[{"given":"Thao T. 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