{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:39:05Z","timestamp":1792809545777,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82341213"],"award-info":[{"award-number":["82341213"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82570278"],"award-info":[{"award-number":["82570278"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Relapsed or refractory B\u2010cell acute lymphoblastic leukemia (R\/R B\u2010ALL) patients exhibit heterogeneous responses to CD19 chimeric antigen receptor T\u2010cell (CAR\u2010T) therapy. However, current studies primarily focus on the CAR\u2010T cells or the leukemia blasts, and the patient's intrinsic molecular characteristics before treatment remain poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifteen R\/R B\u2010ALL patients enrolled in a phase II, single\u2010arm clinical trial (NCT04684147) were stratified at a three\u2010month landmark into 8 good responders and 7 poor responders. Clinical characteristics, genetic alterations and pre\u2010treatment bone marrow transcriptomics were compared between groups, and an exploratory gene\u2010expression score was derived.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Differential expression and enrichment analyses identified a myeloid\u2010like signature and upregulation of myeloid\u2010related genes in poor responders. Seven myeloid\u2010related genes were examined in independent patient cohorts, in B\u2010ALL cell lines and at single\u2010cell resolution in one patient; three were expressed by the leukemic cells themselves and four predominantly by monocytes and macrophages. A separate, variance\u2010derived seven\u2010gene least absolute shrinkage and selection operator (LASSO) score separated response groups in the discovery cohort (apparent area under the curve, AUC 1.00), with bias\u2010corrected and external estimates of approximately 0.57\u20130.59 (external cohort AUC 0.592, 95% confidence interval 0.356\u20130.828).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Pre\u2010treatment transcriptomic profiling revealed a myeloid\u2010like signature in R\/R B\u2010ALL patients with poor CD19 CAR\u2010T response, warranting further investigation as a candidate correlate of treatment outcome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.72395","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:14:43Z","timestamp":1792462483000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome Analysis Identifies a Myeloid\u2010Like Signature in Relapsed or Refractory\n                    <scp>B<\/scp>\n                    \u2010Cell Acute Lymphoblastic Leukemia Patients With Poor Response to\n                    <scp>CD19<\/scp>\n                    Chimeric Antigen Receptor\n                    <scp>T<\/scp>\n                    Cell Therapy"],"prefix":"10.1002","volume":"15","author":[{"given":"Shisen","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Haihe Laboratory of Cell Ecosystem Institute of Hematology and Blood Diseases Hospital, Tianjin Institutes of Health Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4562-5541","authenticated-orcid":false,"given":"Wenbing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Haihe Laboratory of Cell Ecosystem Institute of Hematology and Blood Diseases Hospital, Tianjin Institutes of Health Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7041-8064","authenticated-orcid":false,"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Haihe Laboratory of Cell Ecosystem Institute of Hematology and Blood Diseases Hospital, Tianjin Institutes of Health Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxi","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Haihe Laboratory of Cell Ecosystem Institute of Hematology and Blood Diseases Hospital, Tianjin Institutes of Health Science, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Tianjin Key Laboratory of Cell Therapy for Blood Diseases, Haihe Laboratory of Cell Ecosystem Institute of Hematology and Blood Diseases Hospital, Tianjin Institutes of Health Science, Chinese Academy of Medical Sciences &amp; 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