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This study aims to find the tongue cancer\u2010related E3 ubiquitin ligase. The E3 ubiquitin ligase library was screened. The effect of candidate molecule on tongue cancer was validated through cell viability, cell proliferation, colony formation, invasive assay in vitro, and the xenograft model in vivo. The E3 ubiquitin ligase RNF<\/jats:styled-content>135 significantly promoted the expression of PTEN<\/jats:styled-content> and TP<\/jats:styled-content>53 in SCC<\/jats:styled-content>25 cells. The overexpression of RNF<\/jats:styled-content>135 inhibited the viability, proliferation, and invasion of SCC<\/jats:styled-content>25 cells. Knockdown of RNF<\/jats:styled-content>135 had the opposite effects. Furthermore, RNF<\/jats:styled-content>135 regulates the tumorigenesis activity of SCC<\/jats:styled-content>25 cells in vivo. Our results demonstrated that RNF<\/jats:styled-content>135 had the potential to affect the development of the tongue cancer in vitro. The further in vivo study is helpful to fully understand the role of it.<\/jats:p>","DOI":"10.1002\/cam4.832","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T21:07:21Z","timestamp":1476306441000},"page":"3140-3146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The E3 ubiquitin ligase RNF135 regulates the tumorigenesis activity of tongue cancer SCC25 cells"],"prefix":"10.1002","volume":"5","author":[{"given":"Jian","family":"Jin","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) & Key Laboratory of Oral Biomedicine Ministry of Education School & Hospital of Stomatology Wuhan University Wuhan 430079 China"},{"name":"Department of Oral and Maxillofacial Trauma and Plastic Aesthetic Surgery School & Hospital of Stomatology Wuhan University Wuhan 430079 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