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These animal models require a substantial investment of time and traditionally necessitate the killing of the animal to measure the tumor progression. Several in vivo imaging techniques are being used in both human clinics and preclinical studies, albeit at high cost and requiring particular expertise. Here, we report that the progression of splenomegaly coincides with and positively correlates to colon tumor development in <jats:italic>Apc<\/jats:italic><jats:sup>min716\/+<\/jats:sup> mice expressing a mutant gene encoding an adenomatous polyposis coli protein truncated at amino acid 716. Ultrasound image\u2010based spleen size measurement precisely mirrors splenomegaly development in vivo in the tumor\u2010laden <jats:italic>Apc<\/jats:italic><jats:sup>min716\/+<\/jats:sup> mice. Moreover, the spleen dimensions extracted from the ultrasound sonograms are positively correlated with normalized spleen weight and the number and area of colon tumors. Hence, we propose measuring the spleen size in vivo by ultrasound imaging as a novel approach to estimate splenomegaly development and to indirectly monitor colon tumor development in <jats:italic>Apc<\/jats:italic><jats:sup>min716\/+<\/jats:sup> mice. The widespread use of ultrasound machines in the laboratory setting, coupled with the fact that it is a noninvasive method, make it a straightforward and useful tool for monitoring the experimental progress of colon cancer in mice and determining end points without killing animals strictly for diagnostics purposes.<\/jats:p>","DOI":"10.1002\/cam4.842","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T10:56:56Z","timestamp":1470221816000},"page":"2469-2476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Ultrasound imaging of splenomegaly as a proxy to monitor colon tumor development in <i>Apc<\/i><sup>min716\/+<\/sup> mice"],"prefix":"10.1002","volume":"5","author":[{"given":"Andrea","family":"Hodgson","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Bloomberg School of Public Health Johns Hopkins University  Baltimore Maryland 21025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric M.","family":"Wier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Bloomberg School of Public Health Johns Hopkins University  Baltimore Maryland 21025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Bloomberg School of Public Health Johns Hopkins University  Baltimore Maryland 21025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Bloomberg School of Public Health Johns Hopkins University  Baltimore Maryland 21025"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyi","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Bloomberg School of Public Health Johns Hopkins University  Baltimore Maryland 21025"},{"name":"Department of Oncology and Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University  Baltimore Maryland 21287"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2013.08.020"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21235"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-10-0437"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/548.6506"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pon.3877"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-15-0134"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003840000227"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1293\/tox.2013-0028"},{"key":"e_1_2_7_10_1","first-page":"19","article-title":"Cytokine networks in animal models of colitis\u2010associated cancer","volume":"35","author":"Antoniou E.","year":"2015","journal-title":"Anticancer Res."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2015.04.030"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.06.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2008.03.004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00348.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2012.05.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5382.1509"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/18884"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.09.016"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2009.03.026"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01387.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.04.012"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2441052114"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.061283"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-06-0225"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.377"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-015-3714-3"},{"key":"e_1_2_7_27_1","first-page":"392","article-title":"In vivo imaging of human colon cancer xenografts in immunodeficient mice using a guanylyl cyclase C\u2013specific ligand","volume":"43","author":"Wolfe H. 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