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An effective subunit cholera vaccine that can provide protective immunity with one parenteral immunization would be a major advantage over the existing oral vaccines that can require two doses for optimal protection. The existing vaccines are clearly effective in some settings, but are less so in others, especially with respect to specific groups such as young (2\u20135 years) children. In our efforts to develop a cholera subunit vaccine, we focused on two <jats:italic>Vibrio cholerae<\/jats:italic> antigens, LPS (lipopolysaccharide) and TCP (toxin co\u2010regulated pilus), that are known to induce protective antibodies in animal models and, in the case of anti\u2010LPS antibodies, to be associated with clinical protection of <jats:italic>V. cholerae<\/jats:italic> exposed or vaccinated individuals. This review discusses the current cholera vaccines and compares the advantages of a cholera subunit vaccine to that of the whole cell vaccines. We discuss the possible subunit antigens and prospective targeted use of a subunit cholera vaccine.<\/jats:p>","DOI":"10.1002\/cbdv.200490078","type":"journal-article","created":{"date-parts":[[2004,7,28]],"date-time":"2004-07-28T08:01:29Z","timestamp":1091001689000},"page":"1036-1057","source":"Crossref","is-referenced-by-count":23,"title":["Progress towards Development of a Cholera Subunit Vaccine"],"prefix":"10.1002","volume":"1","author":[{"given":"Ronald\u2005K.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas\u2005J.","family":"Kirn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niranjan","family":"Bose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Stonehouse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shital\u2005A.","family":"Tripathi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavol","family":"Kov\u00e1\u010d","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William\u2005F.","family":"Wade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,28]]},"reference":[{"key":"e_1_2_1_1_2","unstructured":"E.\u2005C. 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