{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T16:55:35Z","timestamp":1758992135793},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,10,29]],"date-time":"2008-10-29T00:00:00Z","timestamp":1225238400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied the cytotoxic effect and the uptake of Pd<jats:sup>II<\/jats:sup> complexes of doxycycline (Dox), [Pd(Dox)Cl<jats:sub>2<\/jats:sub>] (<jats:bold>1<\/jats:bold>), and tetracycline (Tc), [Pd(Tc)Cl<jats:sub>2<\/jats:sub>] (<jats:bold>2<\/jats:bold>), in chronic myelogenous leukemia cells. The effect of the compounds on macrophage viability was also investigated. Compound <jats:bold>1<\/jats:bold> is more effective than compound <jats:bold>2<\/jats:bold> in inhibiting the growth of K562 cells with the <jats:italic>IC<\/jats:italic><jats:sub>50<\/jats:sub> values of 14.44 and 34.54\u2005\u03bc<jats:sc>M<\/jats:sc>, respectively. There is a good correlation between cell\u2010growth inhibition and intracellular metal concentrations, determined by inductively coupled plasma atomic emission spectroscopy (ICP\u2010AES). Incubation of the cells with equitoxic concentrations of both compounds yields approximately the same intracellular Pd concentration. At the <jats:italic>IC<\/jats:italic><jats:sub>50<\/jats:sub> doses, intracellular concentration is <jats:italic>ca.<\/jats:italic> 33\u00d710<jats:sup>\u221216<\/jats:sup> mol\/cell for both compounds <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold>. This suggests that more [Pd(Tc)Cl<jats:sub>2<\/jats:sub>] is needed to produce a cytotoxic effect, because it enters cells more slowly. Both compounds up to 16\u2005\u03bc<jats:sc>M<\/jats:sc> did not affect the viability of mouse peritoneal macrophages after a 48\u2010h incubation. After 72\u2005h of incubation, the <jats:italic>IC<\/jats:italic><jats:sub>50<\/jats:sub> values are 22 for [Pd(Dox)Cl<jats:sub>2<\/jats:sub>] and 40\u2005\u03bc<jats:sc>M<\/jats:sc> for [Pd(Tc)Cl<jats:sub>2<\/jats:sub>]. Therefore, the cytotoxic effect in cancer cells exhibited by both compounds is higher than their effect in macrophages.<\/jats:p>","DOI":"10.1002\/cbdv.200890193","type":"journal-article","created":{"date-parts":[[2008,10,29]],"date-time":"2008-10-29T09:18:23Z","timestamp":1225271903000},"page":"2124-2130","source":"Crossref","is-referenced-by-count":9,"title":["Cytotoxicity and Cellular Accumulation of Palladium(II) Complexes of Tetracyclines"],"prefix":"10.1002","volume":"5","author":[{"given":"Fl\u00e1via\u2005C.\u2005S.","family":"de\u2005Paula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendell","family":"Guerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iara\u2005R.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josianne\u2005N.","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise\u2005Vasconcelos","family":"Botelho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leda\u2005Q.","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elene\u2005C.","family":"Pereira\u2010Maia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,29]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/29.3.245"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-7646(02)00051-1"},{"key":"e_1_2_1_3_2","first-page":"167","volume":"7","author":"Shales D. 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