{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T13:41:03Z","timestamp":1788702063116,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T00:00:00Z","timestamp":1334102400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heme oxygenase\u20101 (HO\u20101) is an inducible antioxidant enzyme that degrades heme to three products, biliverdin, carbon monoxide (CO), and iron ion. The present study was originally designed to characterize the HO\u20101 induction by <jats:italic>Lumbricus<\/jats:italic> extract as a potential cytoprotective mechanism. Through bioactivity\u2010guided fractionation, with human HepG2 cells as the cellular detector, surprisingly, we found that arsenic was enriched in the active fractions isolated from <jats:italic>Lumbricus<\/jats:italic> extract. Arsenic speciation was further carried out by liquid chromatography with inductively coupled plasma mass spectrometry (LC\/ICP\u2010MS). Our results showed that <jats:italic>Lumbricus<\/jats:italic> extract contained two major arsenic species, arsenite (As<jats:sup>III<\/jats:sup>; 53.7%) and arsenate (As<jats:sup>V<\/jats:sup>; 34.2%), and six minor arsenic species. Commercial sodium arsenite (NaAsO<jats:sub>2<\/jats:sub>) was used to verify the effects of <jats:italic>Lumbricus<\/jats:italic> extract on HO\u20101 expression and related intracellular signaling pathways. Both p38 MAP kinase and NF\u2010E2\u2010related factor 2 (Nrf2) pathways were found to modulate HO\u20101 induction by <jats:italic>Lumbricus<\/jats:italic> extract and NaAsO<jats:sub>2<\/jats:sub>. The cytotoxicity of arsenite was augmented by p38 MAP kinase inhibitor SB202190 and HO\u20101 inhibitor tin protoporphyrin IX (SnPP), whereas p38 MAP kinase inhibitor SB202190 also inhibited HO\u20101 induction by NaAsO<jats:sub>2<\/jats:sub>. These results suggest that arsenic\u2010containing compounds are responsible for HO\u20101 induction by <jats:italic>Lumbricus<\/jats:italic> extract. Although the exact role of toxic arsenic compounds in the treatment of oxidative injury remains unclear, concomitant HO\u20101 induction may be a key mechanism to antagonize the cytotoxicity of arsenic compounds in human cells.<\/jats:p>","DOI":"10.1002\/cbdv.201100133","type":"journal-article","created":{"date-parts":[[2012,4,11]],"date-time":"2012-04-11T10:54:42Z","timestamp":1334141682000},"page":"739-754","source":"Crossref","is-referenced-by-count":11,"title":["Concomitant Induction of Heme Oxygenase\u20101 Attenuates the Cytotoxicity of Arsenic Species from <i>Lumbricus<\/i> Extract in Human Liver HepG2 Cells"],"prefix":"10.1002","volume":"9","author":[{"given":"Hongyi","family":"Qi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baowei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. Chris","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Rong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,11]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1039\/b201366p"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0269-7491(03)00047-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1040-8428(01)00215-3"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.10.023"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2003.10.022"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.6-suppl_2-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007276812824"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.9.3354"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.01.014"},{"key":"e_1_2_1_10_2","first-page":"131","volume":"23","author":"Mihara H.","year":"1992","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.58.7.0015"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2005.08.060"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.06.007"},{"key":"e_1_2_1_14_2","first-page":"405","volume":"21","author":"Rong J.","year":"2008","journal-title":"Int. J. Mol. Med."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.01.021"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/152308602760598891"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.37.1.517"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2302004re6"},{"key":"e_1_2_1_19_2","first-page":"1","volume":"105","author":"Balla J.","year":"1992","journal-title":"Trans. Assoc. Am. Physicians"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3029864"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.1688"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2005.7.80"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00297.2003"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2006.8.99"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2009.11.029"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21660"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00046.x"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200201270-00023"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.08.003"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1999.721187.x"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6163"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.20.10925"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI4165"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1039\/a809426h"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200800296"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620220617"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.5.3467"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg141"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.27.6.1124"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)03022-5"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2006.06.005"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-008-9872-9"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.12.2993S"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402004101"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1643"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10968"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg010"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi033"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.11.017"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000007256.44450.8c"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2006.09.020"},{"key":"e_1_2_1_52_2","first-page":"379","volume":"20","author":"Park J. H.","year":"2008","journal-title":"Oncol. Rep."},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2010.01.004"},{"key":"e_1_2_1_54_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2006.8.53"},{"key":"e_1_2_1_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.04.023"},{"key":"e_1_2_1_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx049939e"},{"key":"e_1_2_1_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.03.012"},{"key":"e_1_2_1_58_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.2102"},{"key":"e_1_2_1_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.01.063"},{"key":"e_1_2_1_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01080-08"},{"key":"e_1_2_1_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.12.002"},{"key":"e_1_2_1_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601243"},{"key":"e_1_2_1_63_2","first-page":"3893","volume":"62","author":"Jr. W. H. Miller","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_1_64_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9363"},{"key":"e_1_2_1_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2003.07.009"},{"key":"e_1_2_1_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.15.6798"},{"key":"e_1_2_1_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx700038x"},{"key":"e_1_2_1_68_2","doi-asserted-by":"publisher","DOI":"10.1021\/es0112801"},{"key":"e_1_2_1_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx700076q"},{"key":"e_1_2_1_70_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900006"},{"key":"e_1_2_1_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.06.045"},{"key":"e_1_2_1_72_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.04.022"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbdv.201100133","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.201100133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T11:55:39Z","timestamp":1700567739000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.201100133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":72,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/cbdv.201100133"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.201100133","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}