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The extract was fractionated <jats:italic>via<\/jats:italic> column chromatography, and the resulting fractions were tested for their abilities to quench the fluorescence of the calmodulin (CaM) biosensor <jats:italic>h<\/jats:italic>CaM M124C\u2010<jats:italic>mBBr.<\/jats:italic> From the active fraction, emodin (<jats:bold>1<\/jats:bold>) and <jats:italic>\u03c9<\/jats:italic>\u2010hydroxyemodin (<jats:bold>2<\/jats:bold>) were isolated as CaM inhibitors. Anthraquinones <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> quenched the fluorescence of the <jats:italic>h<\/jats:italic>CaM M124C\u2010<jats:italic>mBBr<\/jats:italic> biosensor in a concentration\u2010dependent manner with <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values of 0.33 and 0.76\u2005\u03bc<jats:sc>M<\/jats:sc>, respectively. The results were compared with those of chlorpromazine (CPZ), a classical inhibitor of CaM, with a <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> value of 1.25\u2005\u03bc<jats:sc>M<\/jats:sc>. Docking analysis revealed that <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> bind to the same pocket of CPZ. The CaM inhibitor properties of <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> were correlated with some of their reported biological properties. Citrinin (<jats:bold>3<\/jats:bold>), methyl 8\u2010hydroxy\u20106\u2010methyl\u20109\u2010oxo\u20109<jats:italic>H<\/jats:italic>\u2010xanthene\u20101\u2010carboxylate (<jats:bold>4<\/jats:bold>), and coniochaetone A (<jats:bold>5<\/jats:bold>) were also isolated in the present study. The X\u2010ray structure of <jats:bold>5<\/jats:bold> is reported for the first time.<\/jats:p>","DOI":"10.1002\/cbdv.201200321","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T10:33:37Z","timestamp":1363257217000},"page":"328-337","source":"Crossref","is-referenced-by-count":8,"title":["Calmodulin Inhibitors from <i>Aspergillus stromatoides<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Mart\u00edn","family":"Gonz\u00e1lez\u2010Andrade","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"Del\u2009Valle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha ;L.","family":"Mac\u00edas\u2010Rubalcava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Sosa\u2010Peinado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Del\u2009Carmen\u2009Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Mata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,13]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(00)01800-6"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20659"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145704004432"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-10-1139"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.6.5.3975"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-008-0097-z"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.233"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm200167g"},{"key":"e_1_2_1_9_2","unstructured":"J. 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