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Its biocontrolling ability was assessed by testing some biochemical attributes such as phosphate<jats:italic>\u2010<\/jats:italic>solubilization, and HCN, NH<jats:sub>3<\/jats:sub>, indole\u20103\u2010acetic acid, and siderophore production. The strain showed positive result for all except indole\u20103\u2010acetic acid, revealing its suitability for a further study. The antibiotic\u2010sensitivity pattern of the strain against 43 antibiotics was also established, which showed resistance to 15 antibiotics. The efficacy of <jats:italic>P. fluorescens<\/jats:italic> strain against grain mold was identified by dual culture technique. Hundred percent inhibition was found against <jats:italic>Fusarium moniliforme<\/jats:italic>, an important causative agent of this disease. The strain was fermented for secondary metabolites and extracted with AcOEt. Chromatographic separation of the extract yielded four known compounds, cyclo(<jats:sc>L<\/jats:sc>\u2010Pro\u2010<jats:sc>L<\/jats:sc>\u2010Phe) (<jats:bold>1<\/jats:bold>), cyclo(<jats:italic>trans<\/jats:italic>\u20104\u2010hydroxy\u2010<jats:sc>L<\/jats:sc>\u2010Pro\u2010<jats:sc>L<\/jats:sc>\u2010Leu) (<jats:bold>2<\/jats:bold>), cyclo(<jats:italic>trans<\/jats:italic>\u20104\u2010hydroxy\u2010<jats:sc>L<\/jats:sc>\u2010Pro\u2010<jats:sc>L<\/jats:sc>\u2010Phe) (<jats:bold>3<\/jats:bold>), and cyclo(Gly\u2010<jats:sc>L<\/jats:sc>\u2010Pro) (<jats:bold>4<\/jats:bold>), which were characterized by spectral analysis and optical rotation. The crude extract, a mixture of <jats:bold>2<\/jats:bold> and <jats:bold>3<\/jats:bold>, and isolated <jats:bold>1<\/jats:bold> were proved to be significantly effective against grain mold fungi. This is the first report on production of these cyclic dipeptides by <jats:italic>P. fluorescens<\/jats:italic> and their antagonistic properties.<\/jats:p>","DOI":"10.1002\/cbdv.201300045","type":"journal-article","created":{"date-parts":[[2014,1,20]],"date-time":"2014-01-20T09:30:12Z","timestamp":1390210212000},"page":"92-100","source":"Crossref","is-referenced-by-count":29,"title":["Isolation and Characterization of Antimicrobial Cyclic Dipeptides from <i>Pseudomonas fluorescens<\/i> and Their Efficacy on Sorghum Grain Mold Fungi"],"prefix":"10.1002","volume":"11","author":[{"given":"Ahil","family":"Sajeli\u2005Begum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaik Ameer","family":"Basha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Govardhanam","family":"Raghavendra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallela Venkata Nagesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukthi","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagannath V.","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhei","family":"Tanemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,20]]},"reference":[{"key":"e_1_2_1_1_2","unstructured":"G. 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