{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T16:44:29Z","timestamp":1758991469508},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T00:00:00Z","timestamp":1418169600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberostemonine stereoisomers are natural alkaloids found in <jats:italic>Stemona tuberosa<\/jats:italic>, that are known to have anti\u2010inflammatory and anti\u2010infective properties. Tuberostemonine alkaloids inhibit inflammation by suppressing the expression of inflammatory mediators such as cyclooxygenase and nitric oxide synthase. However, the direct immunomodulatory properties of tuberostemonine alkaloids in T cells have not been elucidated so far. In this study, the activities in T cells of tuberostemonine N (TbN) and a novel alkaloid, tuberostemonine O (TbO), isolated from <jats:italic>S. tuberosa<\/jats:italic>, were investigated. Although TbN did not have a significant effect on cytokine production in splenic T cells, TbO selectively suppressed interleukin (IL)\u20102 production. Moreover, TbO, but not TbN, significantly inhibited IL\u20102 production by primary CD4<jats:sup>+<\/jats:sup> T cells and delayed the T\u2010cell proliferation in a dose\u2010dependent manner. Addition of excess recombinant IL\u20102 restored the decreased cell\u2010division rates in TbO\u2010treated CD4<jats:sup>+<\/jats:sup> T cells to control levels. Collectively, these findings suggest that the immunomodulatory effects of TbO occurred by the suppression of IL\u20102 expression and IL\u20102\u2010induced T\u2010cell proliferation, suggesting a potential beneficial role of tuberostemonine alkaloids for the control of chronic inflammatory and autoimmune diseases caused by hyperactivated T cells.<\/jats:p>","DOI":"10.1002\/cbdv.201400074","type":"journal-article","created":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T12:33:16Z","timestamp":1418214796000},"page":"1954-1962","source":"Crossref","is-referenced-by-count":8,"title":["Suppression of IL\u20102 Production and Proliferation of CD4<sup>+<\/sup> T Cells by Tuberostemonine O"],"prefix":"10.1002","volume":"11","author":[{"given":"Eun","family":"Jung\u2005Jang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Seo","family":"Kil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye","family":"Ryeon\u2005Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sera","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo","family":"Kyeong\u2005Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi","family":"Gyeong\u2005Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun","family":"Kyoung\u2005Seo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun","family":"Sook\u2005Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,10]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2005-916258"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.02.018"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0205615"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.10.004"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/np900294c"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10286020.2012.757595"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-45100"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1185363"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/np070651"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1099-4831(05)62002-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90564-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2007.03.002"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.5012\/bkcs.2014.35.6.1891"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2010.03289.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051044"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.1.248"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"3502","DOI":"10.4049\/jimmunol.160.7.3502","volume":"160","author":"Zamorano J.","year":"1998","journal-title":"J. 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