{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:38:50Z","timestamp":1783535930102,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T00:00:00Z","timestamp":1475712000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p>The emergence of multidrug resistant pathogens threatened the clinical efficacy of many existing antibiotics. This situation has been recognized globally as a serious concern and justifies further research to discover antimicrobial agents from natural origins including plant extracts. The aim of our work was to evaluate the antimicrobial activities of<jats:italic>Scabiosa arenaria<\/jats:italic><jats:sc>Forssk<\/jats:sc>. extracts and pure compounds using a bioguided fractionation, and try to explain some traditional use of this genus. The best antimicrobial activity\u2010guided fractionation was obtained by Bu<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>fractions of flowers, fruits and (stems and leaves) against<jats:italic>Escherichia coli<\/jats:italic>,<jats:italic>Pseudomonas aeruginosa<\/jats:italic>and<jats:italic>Candida albicans<\/jats:italic>with minimum inhibitory concentration (<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>) values from 0.0195 to 5\u00a0mg\/ml.<jats:italic>Escherichia coli<\/jats:italic>was the most affected bug, thus the<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>of fruits Bu<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>extract showed the best anti\u2010<jats:italic>Escherichia coli<\/jats:italic>activity (<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>\u00a0=\u00a00.0195\u00a0mg\/ml), followed by the (stems and leaves) and flowers Bu<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>extracts;<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>\u00a0=\u00a00.078 and 0.15\u00a0mg\/ml, respectively. Furthermore, the subfractions obtained from these three mixed fractions showed also an important antimicrobial activity against the three microorganisms, with<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>values between 0.0195 and 0.312\u00a0mg\/ml. The fractionation of the aerial part Bu<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>fraction led to the isolation of oleanolic acid (<jats:bold>1<\/jats:bold>) and luteolin 7\u2010<jats:italic>O<\/jats:italic>\u2010glucopyranoside (<jats:bold>2<\/jats:bold>) which are reported here for the first time from<jats:italic>S<\/jats:italic>.\u00a0<jats:italic>arenaria<\/jats:italic>. Both compounds showed good antimicrobial activities with<jats:italic><jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content><\/jats:italic>values ranging from 170 to 683 \u03bc<jats:sc>m<\/jats:sc>and 86 to 347 \u03bc<jats:sc>m<\/jats:sc>, respectively. These results support the use of the<jats:italic>Scabiosa<\/jats:italic>genus to inhibit the growth of tested pathogenic bacteria and yeasts which may reduce illnesses associated with their exposure.<\/jats:p>","DOI":"10.1002\/cbdv.201600028","type":"journal-article","created":{"date-parts":[[2016,7,23]],"date-time":"2016-07-23T14:26:43Z","timestamp":1469284003000},"page":"1262-1272","source":"Crossref","is-referenced-by-count":20,"title":["Antimicrobial Activity of<i>Scabiosa arenaria<\/i><scp>Forssk<\/scp>. Extracts and Pure Compounds Using Bioguided Fractionation"],"prefix":"10.1002","volume":"13","author":[{"given":"Malek","family":"Besbes Hlila","sequence":"first","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biological Active Substances Faculty of Pharmacy University of Monastir Avenue Avicenne 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Mosbah","sequence":"additional","affiliation":[{"name":"Laboratory of Bioresources, Integrative Biology and Exploiting Higher Institute of Biotechnology of Monastir Taher Hadded Avenue 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaouther","family":"Majouli","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Cell Signaling and Disease Research Unit UR 12ES08 Faculty of Medicine of Monastir Avenue Avicenne, 5019 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aymen","family":"Ben Nejma","sequence":"additional","affiliation":[{"name":"Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity Team: Medicinal Chemistry and Natural Products Faculty of Sciences of Monastir University of Monastir Avenue de l\u2019Environnement 5019 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hichem","family":"Ben Jannet","sequence":"additional","affiliation":[{"name":"Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity Team: Medicinal Chemistry and Natural Products Faculty of Sciences of Monastir University of Monastir Avenue de l\u2019Environnement 5019 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Mastouri","sequence":"additional","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biological Active Substances Faculty of Pharmacy University of Monastir Avenue Avicenne 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahjoub","family":"Aouni","sequence":"additional","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biological Active Substances Faculty of Pharmacy University of Monastir Avenue Avicenne 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boulbaba","family":"Selmi","sequence":"additional","affiliation":[{"name":"Laboratory of Bioresources, Integrative Biology and Exploiting Higher Institute of Biotechnology of Monastir Taher Hadded Avenue 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,6]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"138","volume":"3","author":"Usha P. 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