{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T03:43:40Z","timestamp":1771472620650,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,3]],"date-time":"2017-01-03T00:00:00Z","timestamp":1483401600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/20094\u20100"],"award-info":[{"award-number":["2013\/20094\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303349\/2013\u20101"],"award-info":[{"award-number":["303349\/2013\u20101"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>This article reports on the <jats:italic>in vitro<\/jats:italic> activity of the hydroalcoholic extract of <jats:italic>Pfaffia glomerata<\/jats:italic> roots, its hydrolyzed fractions, and pfaffic acid against <jats:italic>Trypanosoma cruzi<\/jats:italic>. The hydroalcoholic extract obtained from dried, milled <jats:italic>P<\/jats:italic>. <jats:italic>glomerata<\/jats:italic> roots was submitted to acid hydrolysis followed by partition with <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>l<jats:sub>3<\/jats:sub>. The concentrated <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>l<jats:sub>3<\/jats:sub> fraction was suspended in Me<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>\/H<jats:sub>2<\/jats:sub>O and partitioned with hexane (<jats:italic>F1<\/jats:italic>), <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>l<jats:sub>3<\/jats:sub> (<jats:italic>F2<\/jats:italic>), and Ac<jats:styled-content style=\"fixed-case\">OE<\/jats:styled-content>t (<jats:italic>F3<\/jats:italic>), in this sequence. The trypanocidal activity of the hydrolyzed extract and its fractions was evaluated <jats:italic>in vitro<\/jats:italic>. The hydroalcoholic extract displayed low activity, but fraction <jats:italic>F1<\/jats:italic> was active against trypomastigotes of the Y strain of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic>, with <jats:italic><jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><\/jats:italic><jats:sub>50<\/jats:sub> = 47.89 \u03bcg\/ml. The steroids campesterol (7.7%), stigmasterol (18.7%), <jats:italic>\u03b2<\/jats:italic>\u2010sitosterol (16.8%), \u0394<jats:sup>7<\/jats:sup>\u2010stigmastenol (4.6%), and \u0394<jats:sup>7<\/jats:sup>\u2010spinasterol (7.5%) were the major constituents of <jats:italic>F1<\/jats:italic>, along with fatty acid esters (7.6%) and eight aliphatic hydrocarbons (30.1%). Fractions <jats:italic>F2<\/jats:italic> and <jats:italic>F3<\/jats:italic> exhibited moderate activity, and pfaffic acid, one of the main chemical constituents of these fractions, displayed <jats:italic><jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><\/jats:italic><jats:sub>50<\/jats:sub> = 44.78 \u03bc<jats:sc>m<\/jats:sc> (21.06 \u03bcg\/ml). On the other hand, the hydroalcoholic extract of <jats:italic>P<\/jats:italic>. <jats:italic>glomerata<\/jats:italic> roots, which is rich in pfaffosides, was inactive. Therefore, the main aglycone of pfaffosides, pfaffic acid, is much more active against trypomastigotes of the Y strain of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> than its corresponding glycosides and should be further investigated.<\/jats:p>","DOI":"10.1002\/cbdv.201600175","type":"journal-article","created":{"date-parts":[[2016,8,22]],"date-time":"2016-08-22T07:33:12Z","timestamp":1471851192000},"source":"Crossref","is-referenced-by-count":6,"title":["<i>In vitro<\/i> Activities of <i>Pfaffia glomerata<\/i> Root Extract, Its Hydrolyzed Fractions and Pfaffic Acid Against <i>Trypanosoma cruzi<\/i> Trypomastigotes"],"prefix":"10.1002","volume":"14","author":[{"given":"M\u00e1rcio L. A. e","family":"Silva","sequence":"first","affiliation":[{"name":"N\u00facleo de Pesquisas em Ci\u00eancias Exatas e Tecnol\u00f3gicas Universidade de Franca Av. 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