{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:21:10Z","timestamp":1761394870722,"version":"3.41.2"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,21]],"date-time":"2017-01-21T00:00:00Z","timestamp":1484956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>Elucidating the origin of life involves synthetic as well as analytical challenges. Herein, for the first time, we describe the use of gel electrophoresis and ultrafiltration to fractionate <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> polymers. Since the first prebiotic synthesis of adenine by <jats:italic>Or\u00f3<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> HCN<\/jats:styled-content> polymers have gained much interest in studies on the origins of life due to the identification of biomonomers and related compounds within them. Here, we demonstrate that macromolecular fractions with electrophoretic mobility can also be detected within <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> polymers. The migration of polymers under the influence of an electric field depends not only on their sizes (one\u2010dimensional electrophoresis) but also their different isoelectric points (two\u2010dimensional electrophoresis, 2\u2010<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>). The same behaviour was observed for several macromolecular fractions detected in <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> polymers. Macromolecular fractions with apparent molecular weights as high as 250 kDa were detected by tricine\u2010<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content> gel electrophoresis. Cationic macromolecular fractions with apparent molecular weights as high as 140 kDa were also detected by 2\u2010<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> polymers synthesized were fractionated by ultrafiltration. As a result, the molecular weight distributions of the macromolecular fractions detected in the <jats:styled-content style=\"fixed-case\">HCN<\/jats:styled-content> polymers directly depended on the synthetic conditions used to produce these polymers. The implications of these results for prebiotic chemistry will be discussed.<\/jats:p>","DOI":"10.1002\/cbdv.201600241","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T15:42:40Z","timestamp":1471016560000},"source":"Crossref","is-referenced-by-count":16,"title":["Detection of Macromolecular Fractions in <scp>HCN<\/scp> Polymers Using Electrophoretic and Ultrafiltration Techniques"],"prefix":"10.1002","volume":"14","author":[{"given":"Margarita R.","family":"Mar\u00edn\u2010Yaseli","sequence":"first","affiliation":[{"name":"Dpto. Evoluci\u00f3n Molecular Centro de Astrobilog\u00eda (INTA\u2010CSIC) Ctra. Torrej\u00f3n\u2010Ajalvir, km 4.2 Torrej\u00f3n de Ardoz, ES\u201028850 Madrid"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Cid","sequence":"additional","affiliation":[{"name":"Dpto. Evoluci\u00f3n Molecular Centro de Astrobilog\u00eda (INTA\u2010CSIC) Ctra. 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