{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T20:17:37Z","timestamp":1788639457251,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,21]],"date-time":"2018-07-21T00:00:00Z","timestamp":1532131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Cancer Institute Cairo University, Cairo, Egypt"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>Breast cancer constitutes the second most prevalent cancer in Egypt, the problem needs more trends in treatment and treatment development either by regimen modification or introducing new drugs, and the main objective of this study is to screen the effects of the aqueous ethanol herbal extract of <jats:italic>Luffa cylindrica<\/jats:italic> leaves on different types of breast cancer cell lines representing different molecular subtypes of the disease. The major active constituents of the extract were tentatively identified by <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> which revealed the presence of phenolic compound derivatives and saponin that may be responsible in part for the activity of the extract. The emphasis was laid on the main apoptotic pathways as well as the extract effect on the normal cell line. Results of phytochemical investigation, cell cycle analysis, and molecular analysis of apoptotic and proliferative markers have shown effective anticancer activity against <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\u20107, <jats:styled-content style=\"fixed-case\">BT<\/jats:styled-content>\u2010474, and <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MB<\/jats:styled-content>\u2010231 cell lines which represent three subtypes of breast cancer, luminal A, luminal B, and triple negative, respectively. On the other hand, the effects on normal lung fibroblast cell line are less prominent at the dose used for treating breast cancer cell lines.<\/jats:p>","DOI":"10.1002\/cbdv.201800045","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T18:06:37Z","timestamp":1528308397000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Chemical Composition of Aqueous Ethanol Extract of <i>Luffa cylindrica<\/i> Leaves and Its Effect on Representation of Caspase\u20108, Caspase\u20103, and the Proliferation Marker Ki67 in Intrinsic Molecular Subtypes of Breast Cancer <i>in Vitro<\/i>"],"prefix":"10.1002","volume":"15","author":[{"given":"Ibrahim M.","family":"Abdel\u2010Salam","sequence":"first","affiliation":[{"name":"Department of Cancer Biology National Cancer Institute Cairo University Kasr Al Eini Street, Fom El Khalig 11796\u2010 Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer M.","family":"Ashmawy","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology National Cancer Institute Cairo University Kasr Al Eini Street, Fom El Khalig 11796\u2010 Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amany M.","family":"Hilal","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology National Cancer Institute Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omayma A.","family":"Eldahshan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Ain Shams University Abbaseyya 1156\u2010 Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Ashour","sequence":"additional","affiliation":[{"name":"Medical Research Department National Institute of Occupational Safety and Health 156 \u2010 EL Hegas Street Cairo\u2010 11351 Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,21]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"971","article-title":"Cancer Incidence in Egypt: Results of the National Population\u2010Based Cancer Registry Program","volume":"43","author":"Ibrahim A. 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