{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:06:49Z","timestamp":1753862809083,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T00:00:00Z","timestamp":1527120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760424"],"award-info":[{"award-number":["81760424"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2016GXNSFAA380254","2014GXNSFAA118172"],"award-info":[{"award-number":["2016GXNSFAA380254","2014GXNSFAA118172"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:p>Blood clots produced by snake\u2010venom thrombin\u2010like enzymes (<jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s) are cleared rapidly, which makes <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s attractive as potential candidates for antithrombotic therapy. We isolated a <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>, agkihpin, from the venom of <jats:italic>Gloydius halys <\/jats:italic><jats:sc>Pallas<\/jats:sc>. Agkihpin was confirmed to a single\u2010chain <jats:styled-content style=\"fixed-case\">TLE<\/jats:styled-content> with molecular mass of 25.5 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> pI<\/jats:styled-content> of 7.43, optimal <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> of 8.0 (hydrolyzing <jats:styled-content style=\"fixed-case\">TAME<\/jats:styled-content>), linked carbohydrate absent, and weak fibrinogen clotting activity. It was also found that <jats:italic>(i) G<\/jats:italic>. <jats:italic>halys<\/jats:italic> might be the latest species in <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s phylogenetic tree; <jats:italic>(ii)<\/jats:italic> different level of conservation was shown among the <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s from the Viperidae snakes. Some of those site may account for different activities exhibited by those <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s, especially position 181, at which a fibrinogenolytic activity increase was found when a basic and larger amino acid substituted by a neutral and smaller one; <jats:italic>(iii)<\/jats:italic> an extra <jats:italic>\u03b1<\/jats:italic>\u2010helix constructed with a \u2018Pro + acidic amino acid + aromatic amino acid\u2019 pattern was found in the <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s from <jats:italic>Gloydius<\/jats:italic> and <jats:italic>Agkistrodon<\/jats:italic> snakes, although it does not necessarily imply an effect on the fibrinogenolytic activity of the <jats:styled-content style=\"fixed-case\">SVTLE<\/jats:styled-content>s. This study provided some new insight into the activity of SVTLE.<\/jats:p>","DOI":"10.1002\/cbdv.201800122","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T06:01:20Z","timestamp":1524722480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Agkihpin, a Distinct <scp>SVTLE<\/scp> from the Venom of <i>Gloydius halys <\/i><scp>Pallas<\/scp>: Purification, Characterization and Structure\u2013Activity Determination"],"prefix":"10.1002","volume":"15","author":[{"given":"Yulu","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Genetics School of Pre\u2010clinical Medicine Guangxi Medical University 22 Shuangyong Road Nanning 530021 P. R. 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