{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T21:03:34Z","timestamp":1762376614959,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T00:00:00Z","timestamp":1572825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005283","name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Cissampelos sympodialis<\/jats:italic> <jats:sc>Eichler<\/jats:sc> is well studied and investigated for its antiasthmatic properties, but there are no data in the literature describing antibacterial properties of alkaloids isolated from this botanical species. This work reports the isolation and characterization of phanostenine obtained from roots of <jats:italic>C. sympodialis<\/jats:italic> and describes for the first time its antimicrobial and antibiotic modulatory properties. Phanostenine was first isolated from <jats:italic>Cissampelos sympodialis<\/jats:italic> and its antibacterial activities were determined. Chemical structures of the alkaloid isolate were determined using spectroscopic and chemical analyses. Phanostenine was also tested for its antibacterial activity against standard strains and clinical isolates of <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic>. Minimal inhibitory concentration (MIC) was determined in a microdilution assay and for the evaluation of antibiotic resistance\u2010modifying activity. MIC of the antibiotics was determined in the presence or absence of phanostenine at sub\u2010inhibitory concentrations. The evaluation of antibacterial activity by microdilution assay showed activity for all strains with better values against <jats:italic>S. aureus<\/jats:italic> ATCC 12692 and <jats:italic>E. coli<\/jats:italic> 27 (787.69\u2005m<jats:sc>m<\/jats:sc>). The evaluation of aminoglycoside antibiotic resistance\u2010modifying activity showed reduction in the MIC of the aminoglycosides (amikacin, gentamicin and neomycin) when associated with phanostenine, MIC reduction of antibiotics ranging from 21\u2009% to 80\u2009%. The data demonstrated that phanostenine possesses a relevant ability to modify the antibiotic activity <jats:italic>in\u2005vitro<\/jats:italic>. We can suggest that phanostenine presents itself as a promising tool as an adjuvant for novel antibiotics formulations against bacterial resistance.<\/jats:p>","DOI":"10.1002\/cbdv.201900313","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T20:04:31Z","timestamp":1569269071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antibiotic\u2010Potentiating Activity of Phanostenine Isolated from <i>Cissampelos sympodialis<\/i><scp>Eichler<\/scp>"],"prefix":"10.1002","volume":"16","author":[{"given":"Walmir E. M.","family":"Cunha","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Laborat\u00f3rio de Pesquisa de Produtos Naturais Universidade Regional do Cariri  Av. Cel. Ant\u00f4nio Luiz, 1161, Pimenta 63105-000 Crato, CE Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cicera J.","family":"Camilo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Etnobiologia e Conserva\u00e7\u00e3o da Natureza, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"de F. A. Nonato","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Etnobiologia e Conserva\u00e7\u00e3o da Natureza, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnatan W. S.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica Biol\u00f3gica, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia K. G.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica Biol\u00f3gica, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique D. M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Laborat\u00f3rio de Pesquisa de Produtos Naturais Universidade Regional do Cariri  Av. Cel. Ant\u00f4nio Luiz, 1161, Pimenta 63105-000 Crato, CE Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Etnobiologia e Conserva\u00e7\u00e3o da Natureza, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica Biol\u00f3gica, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irwin R. A.","family":"Menezes","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Laborat\u00f3rio de Pesquisa de Produtos Naturais Universidade Regional do Cariri  Av. Cel. Ant\u00f4nio Luiz, 1161, Pimenta 63105-000 Crato, CE Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Etnobiologia e Conserva\u00e7\u00e3o da Natureza, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Qu\u00edmica Biol\u00f3gica, Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri  63105-000 Crato, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Telma L. G.","family":"de Lemos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotransforma\u00e7\u00e3o e Produtos Naturais Universidade Federal do Cear\u00e1  60455-970 Fortaleza, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimundo","family":"Braz\u2010Filho","sequence":"additional","affiliation":[{"name":"Universidade Estadual do Norte Fluminense Darcy Ribeiro Setor de Qu\u00edmica de Produtos Naturais  28013-602 Campos dos Goytacazes, RJ Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola F. G.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Laborat\u00f3rio de Pesquisa de Produtos Naturais Universidade Regional do Cariri  Av. Cel. Ant\u00f4nio Luiz, 1161, Pimenta 63105-000 Crato, CE Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ensino em Sa\u00fade Centro Universit\u00e1rio Dr. Le\u00e3o Sampaio  63040-005 Juazeiro do Norte, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edinardo F. F.","family":"Matias","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ensino em Sa\u00fade Centro Universit\u00e1rio Dr. Le\u00e3o Sampaio  63040-005 Juazeiro do Norte, CE Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Selcuk University, Faculty of Science Department of Biology, Campus  42130 Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 G. 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