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Herein, we report the isolation of phytoconstituents from <jats:italic>Piper cubeba<\/jats:italic> fruit methanol extract and investigated their efflux pump inhibitory potential against <jats:italic>S. aureus<\/jats:italic> MsrA pump. Four isolated compounds, viz. pellitorine, sesamin, piperic acid and tetrahydropiperine studied in combination with erythromycin in <jats:italic>S. aureus<\/jats:italic> RN4220, exhibited 2\u20138\u2010fold reduction in minimum inhibitory concentration (MIC) of erythromycin. Pellitorine and sesamin decreased MIC of erythromycin by 8\u2010fold. The real\u2010time fluorometry\u2010based efflux and accumulation studies of ethidium bromide (EtBr) on <jats:italic>S. aureus<\/jats:italic> RN4220 in the presence of these compounds showed reduced efflux and enhanced uptake, thus indicating inhibition of the efflux pump. Pellitorine showed significant post\u2010antibiotic effect of erythromycin. The results revealed that the primary mechanism of action of these compounds involves steady ATP production impairment.<\/jats:p>","DOI":"10.1002\/cbdv.202000144","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T01:59:30Z","timestamp":1590371970000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["MsrA Efflux Pump Inhibitory Activity of <i>Piper cubeba<\/i> L.f. and its Phytoconstituents against <i>Staphylococcus aureus<\/i> RN4220"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3178-2992","authenticated-orcid":false,"given":"Pallavi","family":"Ahirrao","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry Chandigarh College of Pharmacy  Landran-Mohali 140307 Punjab India"},{"name":"Research scholar of IK Gujral Punjab Technical University Kapurthala Department of Pharmaceutical Chemistry Chandigarh College of Pharmacy  CGC Landran, Mohali 140307 Punjab India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rushikesh","family":"Tambat","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Bioactive Screening Laboratory CSIR-Institute of Microbial Technology, Sector 39-A  Chandigarh 160036 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishtha","family":"Chandal","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Bioactive Screening Laboratory CSIR-Institute of Microbial Technology, Sector 39-A  Chandigarh 160036 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisha","family":"Mahey","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Bioactive Screening Laboratory CSIR-Institute of Microbial Technology, Sector 39-A  Chandigarh 160036 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjoo","family":"Kamboj","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Chandigarh College of Pharmacy  Landran-Mohali 140307 Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upendra K.","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Chandigarh College of Pharmacy  Landran-Mohali 140307 Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inder Pal","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Natural Products National Institute of Pharmaceutical Education and Research (NIPER), Sector-67  SAS Nagar, Mohali 160062 Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay M.","family":"Jachak","sequence":"additional","affiliation":[{"name":"Department of Natural Products National Institute of Pharmaceutical Education and Research (NIPER), Sector-67  SAS Nagar, Mohali 160062 Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemraj S.","family":"Nandanwar","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Bioactive Screening Laboratory CSIR-Institute of Microbial Technology, Sector 39-A  Chandigarh 160036 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00081-09"},{"key":"e_1_2_8_2_1","first-page":"59","article-title":"\u2018Multidrug efflux pumps in Staphylococcus aureus: an update\u2019","volume":"7","author":"Costa S. 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