{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:07:01Z","timestamp":1753862821643,"version":"3.41.2"},"reference-count":61,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human Islet Amyloid Polypeptide (hIAPP) plays a key role in the pathogenesis of type II diabetes. The aim of this research was to search for new amyloidogenic fragments of hIAPP. An initial attempt to predict the amyloidogenic cores of polypeptides\/proteins using five different computer programs did not provide conclusive results. Therefore, we synthesized hIAPP fragments covering the entire hormone. The fragments were assessed for their aggregation ability, using recommended methods to search for the amyloidogenic fragments of the polypeptides\/proteins. It was found that fragments (18\u201322) H\u2010HSSNN\u2010OH and (33\u201337) H\u2010GSNTY\u2010NH<jats:sub>2<\/jats:sub> aggregate and form stable amyloid\u2010like structures. Both of these fragments have a much higher antiproliferative activity relative to the RIN\u20105F cell compared to the (23\u201327) H\u2010FGAIL\u2010OH fragment widely regarded as the amyloidogenic core of amylin. The analog of (33\u201337) H\u2010GSNTY\u2010NH<jats:sub>2<\/jats:sub> containing a free carboxy group on the C\u2010terminal amino acid (H\u2010GSNTY\u2010OH) does not have amyloidogenic properties and can therefore be considered as a potential inhibitor of amylin aggregation. Research on the use of non\u2010aggregating amylin fragments as potential hormone aggregation inhibitors is ongoing.<\/jats:p>","DOI":"10.1002\/cbdv.202000501","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T13:37:51Z","timestamp":1599053871000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["New Human Islet Amyloid Polypeptide Fragments Susceptible to Aggregation"],"prefix":"10.1002","volume":"17","author":[{"given":"Kamil","family":"Rozniakowski","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 Lodz 90-924 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Fraczyk","sequence":"additional","affiliation":[{"name":"Institute of Applied Computer Science Lodz University of Technology  Stefanowskiego \u0141\u00f3d\u017a, 18\/22 Lodz 90-537 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krystian","family":"Galecki","sequence":"additional","affiliation":[{"name":"Institute of General Food Chemistry Faculty of Biotechnology and Food Sciences Lodz University of Technology  Stefanowskiego 4\/10 Lodz 90-924 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Wietrzyk","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences  12 Rudolfa Weigla St. 53-114 Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Filip\u2010Psurska","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences  12 Rudolfa Weigla St. 53-114 Wroclaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Fraczyk","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 Lodz 90-924 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-3362","authenticated-orcid":false,"given":"Zbigniew J.","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 Lodz 90-924 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4581-947X","authenticated-orcid":false,"given":"Beata","family":"Kolesinska","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 Lodz 90-924 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,8]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8628"},{"key":"e_1_2_7_2_1","unstructured":"International Diabetes Federation. IDF Diabetes Atlas 8th edn. Brussels Belgium: International Diabetes Federation 2017. http:\/\/www.diabetesatlas.org; last accessed on January 23 2019."},{"key":"e_1_2_7_3_1","unstructured":"W.\u2005F. Ganong \u2018Review of Medical Physiology\u2019 McGraw-Hill Appleton & Lange 21st edition 2003."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2001)60:6<438::AID-BIP10182>3.0.CO;2-A"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.12.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/03009734.2011.573884"},{"key":"e_1_2_7_7_1","first-page":"645","article-title":"\u2018From Amyloidogenic Peptides to Low-Molecular Supramolecular Structures\u2019","volume":"64","author":"Rozniakowski K. K.","year":"2010","journal-title":"Wiad. Chem."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-045115"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP14037.CR"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp903106x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305517110"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1102356108"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-17-0105"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2007.01.007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5036"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3422"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.25.15458"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0442hyp"},{"key":"e_1_2_7_19_1","unstructured":"http:\/\/prowl.rockefeller.edu\/aainfo\/struct.htm."},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10610270412331328943"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja710484d"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102883200"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4593"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3286"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-009-0572-4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062486907"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(01)00078-3"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","unstructured":"F. Rahimi G. Bitan in \u2018Non-fibrillar Amyloidogenic Protein Assemblies - Common Cytotoxins Underlying Degenerative Diseases\u2019 Eds. F. Rahimi G. Bitan Springer Dordrecht 2012 pp. 1\u201336.","DOI":"10.1007\/978-94-007-2774-8_1"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200703133"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200703133"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-8477(02)00630-5"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805957106"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2000.4268"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.16.1.57"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200904902"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200904902"},{"key":"e_1_2_7_35_1","first-page":"910","article-title":"\u2018A search for Amyloidogenic Regions in Protein Chain\u2019","volume":"40","author":"Galzitskaia O. V.","year":"2006","journal-title":"Mol. Biol."},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp691"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2014.06.007"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzm042"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku399"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1432"},{"key":"e_1_2_7_41_1","first-page":"47","article-title":"\u2018Identification of Amyloidogenic Regions in the Spine of Insulin Fibrils\u2019","volume":"84","author":"Surin A. K.","year":"2019","journal-title":"Biochemistry"},{"key":"e_1_2_7_42_1","first-page":"117","article-title":"\u2018AGGRESCAN: a Server for the Prediction and Evaluation of \u2018Hot Spots\u2019 of Aggregation in Polypeptides\u2019","volume":"8","author":"ConchilloSole O.","year":"2007","journal-title":"BMC Bioinf."},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201402862"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201906908"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.4236\/jbpc.2010.13018"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002867107"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.8.2666510"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2933"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12154-009-0027-5"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzp059"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11426-011-4451-3"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.3.491"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.0020177"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336896.2015.1044186"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2005.01.004"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003291"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-465-0_14"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201800543"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201900339"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202000501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202000501","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202000501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T07:09:11Z","timestamp":1693897751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202000501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":61,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/cbdv.202000501"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202000501","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"type":"print","value":"1612-1872"},{"type":"electronic","value":"1612-1880"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"2020-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2000501"}}