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The ME obtained by maceration of the propolis sample was fractionated with solvents of different polarities and then, purified by silica gel column chromatography through biomonitoring of its antimicrobial activity against mollicute strains. Analysis by gas chromatography\u2010mass spectrometry (GC\/MS) enabled the identification of compounds using the NIST library. Minimum inhibitory concentrations (MICs) of the samples were determined by broth microdilution. Anti\u2010adhesive assays were performed with <jats:italic>Mycoplasma pneumoniae<\/jats:italic> cells. The hexane (MIC=62.5\u2005mg\/L) and dichloromethane (MIC=125\u2005mg\/L) fractions presented the most promising results against <jats:italic>M. pneumoniae<\/jats:italic>. They were fractionated into 74 subfractions, and even the best ones did not show better results (MIC&gt;250\u2005mg\/L) than their original fractions, likely due to the loss of terpene compounds that seem to act in synergy. The dichloromethane subfraction FD4 was highlighted in the anti\u2010adhesive assay with an inhibitory activity of 21.6\u2009%. A synergistic effect of the nonpolar compounds in <jats:italic>M. quadrifasciata<\/jats:italic> propolis may be responsible for its antibacterial activity, but several purified components can improve its anti\u2010adhesive properties.<\/jats:p>","DOI":"10.1002\/cbdv.202000711","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T03:30:11Z","timestamp":1605583811000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mollicute Anti\u2010Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee <i>Melipona quadrifasciata<\/i>"],"prefix":"10.1002","volume":"18","author":[{"given":"Caroline","family":"Galgowski","sequence":"first","affiliation":[{"name":"Graduation Program in Chemistry University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Pavanelo Frare","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences University of Blumenau  Rua S\u00e3o Paulo 2171, Campus 3 89030-001 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martinho","family":"Rau","sequence":"additional","affiliation":[{"name":"Graduation Program in Chemistry University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"},{"name":"Department of Chemistry University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Debiase Alberton","sequence":"additional","affiliation":[{"name":"Graduation Program in Chemistry University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"},{"name":"Department of Pharmaceutical Sciences University of Blumenau  Rua S\u00e3o Paulo 2171, Campus 3 89030-001 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9rgio","family":"Althoff","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Guedes","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Blumenau  Rua S\u00e3o Paulo 2171, Campus 3 89030-001 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caio Mauricio","family":"Mendes de Cordova","sequence":"additional","affiliation":[{"name":"Graduation Program in Chemistry University of Blumenau  Rua Antonio da Veiga 140 89030-903 Blumenau, SC Brazil"},{"name":"Department of Pharmaceutical Sciences University of Blumenau  Rua S\u00e3o Paulo 2171, Campus 3 89030-001 Blumenau, SC Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"C.\u2005M.\u2005O. 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