{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:29:41Z","timestamp":1791930581645,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009894","name":"Politechnika L\u00f3dzka","doi-asserted-by":"publisher","award":["501-3-32-1-4"],"award-info":[{"award-number":["501-3-32-1-4"]}],"id":[{"id":"10.13039\/501100009894","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amylin (hIAPP) aggregation leads to the formation of insoluble deposits and is one of the factors in the development of type II diabetes. The aim of this research was to find <jats:italic>N\u2010<\/jats:italic>methylated analogs of the aggregating amylin fragments 18\u201322, 23\u201327, and 33\u201337, which would not themselves be susceptible to aggregation and would inhibit the aggregation of the amyloidogenic cores of the hormone. None of the analogs of fragment 18\u201322 containing one or two <jats:italic>N<\/jats:italic>\u2010methylated amino acid residues showed any tendency to aggregate. Only the peptide H\u2212F(<jats:italic>N<\/jats:italic>\u2212Me)GA(<jats:italic>N<\/jats:italic>\u2212Me) IL\u2212OH (<jats:bold>6<\/jats:bold>) derived from the 23\u201327 hIAPP hot spot did not form fibrous structures. All analogs of the 33\u201337 amylin fragment were characterized by the ability to form aggregates, despite the presence of <jats:italic>N<\/jats:italic>\u2010methylated amino acids in their structures. <jats:italic>N<\/jats:italic>\u2010Methylated peptides <jats:bold>1<\/jats:bold>\u2013<jats:bold>5<\/jats:bold> demonstrated inhibitory properties against the aggregation of fragment 18\u201322. Aggregation of the amyloidogenic core of 23\u201327 was significantly inhibited by <jats:italic>N<\/jats:italic>\u2010methylated peptides <jats:bold>1<\/jats:bold>\u2013<jats:bold>3<\/jats:bold> derived from the (18\u201322) H\u2212HSSNN\u2212OH fragment and by the H\u2212F(<jats:italic>N<\/jats:italic>\u2010Me)GA(<jats:italic>N<\/jats:italic>\u2212Me)IL\u2212OH (<jats:bold>6<\/jats:bold>) fragment derived from the 23\u201327 amylin hot spot. Fragment (33\u201337) H\u2212GSNTY\u2212NH<jats:sub>2<\/jats:sub> was found to be inhibited in the presence of <jats:italic>N<\/jats:italic>\u2010methylated peptides <jats:bold>1<\/jats:bold>\u2013<jats:bold>3<\/jats:bold> derived from the 18\u201322 fragment and by the double methylated peptide H\u2212F(<jats:italic>N<\/jats:italic>\u2212Me)GA(<jats:italic>N<\/jats:italic>\u2212Me)IL\u2212OH (<jats:bold>6<\/jats:bold>). Research on the possibility of using <jats:italic>N<\/jats:italic>\u2010methylated analogs of amyloidogenic amylin cores as inhibitors of hormone aggregation is ongoing, with a focus on finding the minimum concentration of <jats:italic>N<\/jats:italic>\u2010methylated peptides capable of inhibiting the aggregation of hIAPP hot spots.<\/jats:p>","DOI":"10.1002\/cbdv.202000842","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T07:53:26Z","timestamp":1608278006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>N<\/i>\u2010Methylated Analogs of hIAPP Fragments 18\u201322, 23\u201327, 33\u201337 Inhibit Aggregation of the Amyloidogenic Core of the Hormone"],"prefix":"10.1002","volume":"18","author":[{"given":"Kamil","family":"Rozniakowski","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 90-924 Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystian","family":"Galecki","sequence":"additional","affiliation":[{"name":"Institute of General Food Chemistry, Faculty of Biotechnology &amp; Food Sciences Lodz University of Technology  Stefanowskiego 4\/10 90-924 Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Wietrzyk","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences  12 Rudolfa Weigla St. 53-114 Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Filip\u2010Psurska","sequence":"additional","affiliation":[{"name":"Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences  12 Rudolfa Weigla St. 53-114 Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Fraczyk","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 90-924 Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbigniew J.","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 90-924 Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4581-947X","authenticated-orcid":false,"given":"Beata","family":"Kolesinska","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry, Faculty of Chemistry Lodz University of Technology  Zeromskiego 116 90-924 Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2005.00318.x"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063522"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206593200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2004.06.008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800828u"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-008-1255-x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400260200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi020314u"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200800762"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.03.022"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100143"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511000316"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.09.037"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05648"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20020118)41:2<257::AID-ANIE257>3.0.CO;2-M"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2010.02.008"},{"key":"e_1_2_7_17_1","first-page":"1749","article-title":"Reduction in Aggregation of Insulin Through Conformational Stabilization by Glucose\u2019","volume":"19","author":"Barzegar A.","year":"2007","journal-title":"Asian J. Chem."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3765(20020916)8:18<4285::AID-CHEM4285>3.0.CO;2-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm901092h"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003554200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja075356q"},{"key":"e_1_2_7_22_1","first-page":"826706","article-title":"Amylin Uncovered: A Review on the Polypeptide Responsible for Type II Diabetes\u2019","author":"Pillay K.","year":"2013","journal-title":"BioMed Res. Int."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.23.11138"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2510208.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2005.05.010"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200604056"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5244"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65360-6"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507471103"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2010.01.006"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202000501"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.201402862"},{"key":"e_1_2_7_33_1","first-page":"153","article-title":"Spectroscopic and Calorimetric Studies of Congo Red Dye-Amyloid \u03b2 Peptide Complexes\u2019","volume":"1","author":"Yokoyama K.","year":"2010","journal-title":"J. Biol. Phys. Chem."},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002867107"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2011.01110.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.8.2666510"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24203706"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201800543"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24081600"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201900339"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202000842","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202000842","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202000842","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T06:10:41Z","timestamp":1693721441000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202000842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,17]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/cbdv.202000842"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202000842","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,17]]},"assertion":[{"value":"2020-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2000842"}}