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The optimum conditions (2\u2009% cellulase, 47\u2009\u00b0C for 2.5\u2005h, then, 95\u2009\u00b0C for 2.5\u2005h, pH\u20054.1, solid\/liquid ratio 1\u2009:\u20097.6) were identified by a combination of single factor optimization and response surface DOE (design of experiment) methods, and achieved a yield of 4.7\u2009%. Treatment with 1\u2009% TCA for 10\u2005min, then, 2\u2009% DEAE\u2010cellulose removed protein and colored impurities. Purified VAP retained most of the radical\u2010scavenging activities and GES\u20101\u2005cell protection capability <jats:italic>in\u2005vitro<\/jats:italic>, indicating VAP were the key active components of <jats:italic>V. axillare<\/jats:italic>. Some molecular features were identified by FT\u2010IR and NMR analyses. The molecular weight was estimated from DOSY NMR experiments to be around 21\u2005kDa. There were 6.3\u2009% uronic acid residues in the VAP. The constituent sugars after TFA hydrolysis were identified by HPLC to include glucose, arabinose, rhamnose, galactose, and xylose in a molar ratio of 405\u2009:\u2009259\u2009:\u200982\u2009:\u200942\u2009:\u20091.<\/jats:p>","DOI":"10.1002\/cbdv.202000864","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T00:02:51Z","timestamp":1612569771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Optimization of Extraction and Purification of Polysaccharides from <i>Veronicastrum axillare<\/i>, and Evaluation of Their Biological Activities"],"prefix":"10.1002","volume":"18","author":[{"given":"Yijia","family":"Qi","sequence":"first","affiliation":[{"name":"College of Life Sciences Zhejiang Chinese Medical University  Hangzhou 310053 P. R. 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