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The chimeras were obtained with high purity, demonstrating their synthetic viability. The chimeras\u2019 antibacterial activity against Gram\u2010positive and Gram\u2010negative strains was evaluated. Our results showed that all the chimeras exhibited greater antibacterial activity against the evaluated strains than the individual sequences, suggesting that chemical binding of short sequences derived from AMPs significantly increased the antibacterial activity. For each strain, the chimera with the best antibacterial activity exerted a bacteriostatic and\/or bactericidal effect, which was dependent on the concentration. It was found that: (i) the antibacterial activity of a chimera is mainly influenced by the linked sequences, the palindromic motif RLLRRLLR being the most relevant one; (ii) the inclusion of a spacer between the short sequences did not significantly affect the chimera's synthesis process; however, it enhanced its antibacterial activity against Gram\u2010negative and Gram\u2010positive strains; on the other hand, (iii) the replacement of Arg with Lys in the LfcinB or BFII sequences improved the chimeras\u2019 synthesis process without significantly affecting their antibacterial activity. These results illustrate the great importance of the synthesis of chimeric peptides for the generation of promising antibacterial peptides.<\/jats:p>","DOI":"10.1002\/cbdv.202000885","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T04:39:05Z","timestamp":1608871145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Designing Chimeric Peptides: A Powerful Tool for Enhancing Antibacterial Activity"],"prefix":"10.1002","volume":"18","author":[{"given":"H\u00e9ctor Manuel","family":"Pineda\u2010Casta\u00f1eda","sequence":"first","affiliation":[{"name":"Chemistry Department Universidad Nacional de Colombia  Carrera 45 No. 26\u201385, Building 451, Office 409 Bogot\u00e1 Zip Code 11321 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin Andrey","family":"Huertas\u2010Ortiz","sequence":"additional","affiliation":[{"name":"Chemistry Department Universidad Nacional de Colombia  Carrera 45 No. 26\u201385, Building 451, Office 409 Bogot\u00e1 Zip Code 11321 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aura Luc\u00eda","family":"Leal\u2010Castro","sequence":"additional","affiliation":[{"name":"Medicine Faculty Universidad Nacional de Colombia  Carrera 45 No. 26\u201385, Building 451, Office 409 Bogot\u00e1 Zip Code 11321 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yerly","family":"Vargas\u2010Casanova","sequence":"additional","affiliation":[{"name":"Microbiology Department Pontificia Universidad Javeriana  Carrera 7 No. 40\u201362 Bogot\u00e1 Zip Code 110231 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Marcela","family":"Parra\u2010Giraldo","sequence":"additional","affiliation":[{"name":"Microbiology Department Pontificia Universidad Javeriana  Carrera 7 No. 40\u201362 Bogot\u00e1 Zip Code 110231 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier Eduardo","family":"Garc\u00eda\u2010Casta\u00f1eda","sequence":"additional","affiliation":[{"name":"Pharmacy Department Universidad Nacional de Colombia, Carrera  45 No. 26\u201385, Building 450, Office 213 Bogot\u00e1 Zip Code 11321 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuly Jenny","family":"Rivera\u2010Monroy","sequence":"additional","affiliation":[{"name":"Chemistry Department Universidad Nacional de Colombia  Carrera 45 No. 26\u201385, Building 451, Office 409 Bogot\u00e1 Zip Code 11321 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,19]]},"reference":[{"key":"e_1_2_7_1_1","unstructured":"\u2018WHO | WHO publishes list of bacteria for which new antibiotics are urgently needed\u2019 can be found under http:\/\/www.who.int\/mediacentre\/news\/releases\/2017\/bacteria-antibiotics-needed\/en\/ 2017."},{"key":"e_1_2_7_2_1","unstructured":"\u2018WHO | World Health Organization\u2019 can be found under http:\/\/www.who.int\/antimicrobial-resistance\/Microbes_and_Antimicrobials\/en\/ 2016."},{"key":"e_1_2_7_3_1","first-page":"16","article-title":"P\u00e9ptidos antimicrobianos: p\u00e9ptidos con m\u00faltiples funciones\u2019","volume":"18","author":"Casta\u00f1eda-Casimiro J.","year":"2009","journal-title":"Alergia, asma e Inmunol."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.639"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-014-9753-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.09.032"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2016-0175"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5373-z"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(03)00152-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09482.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22030452"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22060987"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22101641"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00329"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8159"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.150518097"},{"key":"e_1_2_7_17_1","first-page":"1","article-title":"Buforins: Histone H2\u2005A-derived antimicrobial peptides from toad stomach\u2019","volume":"1788","author":"Hyun J.","year":"2009","journal-title":"BBA-Biomembranes"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.01.015"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.201903834"},{"key":"e_1_2_7_20_1","unstructured":"P. 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