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However, the protective effect on immunological liver injury (ILI) of <jats:italic>V. yedoensis<\/jats:italic> had been rarely reported. This study aimed to explore the protective effect of <jats:italic>n<\/jats:italic>\u2010butanol extract (BE) from <jats:italic>V. yedoensis<\/jats:italic> on ILI <jats:italic>in\u2005vitro<\/jats:italic> and <jats:italic>in\u2005vivo. In vitro<\/jats:italic>, the BE significantly inhibited the secretions of Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg) in the HepG2.2.15 cells and the replication of HBV DNA. The research data <jats:italic>in\u2005vivo<\/jats:italic> revealed that the BE reduced the levels of alanine transaminase (ALT), aspartate transaminase (AST), and methane dicarboxylic aldehyde (MDA) in liver tissues of the ConA\u2010induced mice, while increased the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH\u2010PX), and the effective contents of tumor necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic> (TNF\u2010<jats:italic>\u03b1<\/jats:italic>), interferon\u2010<jats:italic>\u03b3<\/jats:italic> (IFN\u2010<jats:italic>\u03b3<\/jats:italic>) and the BE could ameliorate liver histological lesions. These results motivated a further investigation into the chemical constituents of BE. Four coumarins (esculetin, prionanthoside, cichoriin, and esculin) and one flavonoid (quercetin\u20103\u2010<jats:italic>O<\/jats:italic>\u2010galactoside) were isolated from the BE by silica gel column chromatography and recrystallization, of which structures were eventually confirmed by <jats:sup>1<\/jats:sup>H\u2010NMR, <jats:sup>13<\/jats:sup>C\u2010NMR, and MS.<\/jats:p>","DOI":"10.1002\/cbdv.202001043","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T14:02:44Z","timestamp":1619791364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Protective Effect of <i>n<\/i>\u2010Butanol Extract from <i>Viola yedoensis<\/i> on Immunological Liver Injury"],"prefix":"10.1002","volume":"18","author":[{"given":"Haiqing","family":"Chu","sequence":"first","affiliation":[{"name":"College of Pharmacy Ningxia Medical University  1160 Shengli Street Yinchuan 750004 P. R. 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