{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:18:09Z","timestamp":1772824689542,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:00:00Z","timestamp":1618358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070017"],"award-info":[{"award-number":["32070017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770017"],"award-info":[{"award-number":["31770017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32011530016"],"award-info":[{"award-number":["32011530016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012591","name":"Program for Liaoning Innovative Talents in University","doi-asserted-by":"publisher","award":["LD2020002"],"award-info":[{"award-number":["LD2020002"]}],"id":[{"id":"10.13039\/501100012591","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2019-ZD-0198"],"award-info":[{"award-number":["2019-ZD-0198"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["20170520258"],"award-info":[{"award-number":["20170520258"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Breast cancer is one of the most common cancer with high morbidity and mortality in women. This study aimed to explore the potential mechanism of costunolide inducing MCF\u20107 cells apoptosis by multi\u2010spectroscopy, molecular docking, and cell experiments. The results manifested that costunolide interacted with calf thymus DNA (ct\u2010DNA) in a spontaneous manner, and the minor groove as the preferential binding mode. Furthermore, costunolide inhibited cell proliferation and colony formation. Hoechst 33258 staining showed that cell apoptosis induced by costunolide might be related to DNA damage. The apoptosis mechanism relied on regulating the protein expression of Bax, Bcl\u20102, p53, Caspase\u20103 and the activation of p38MAPK and nuclear factor \u03baB (NF\u2010\u03baB) pathways. This study will provide some experimental basis and potential therapeutic strategy for breast cancer treatment.<\/jats:p>","DOI":"10.1002\/cbdv.202001069","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T21:53:14Z","timestamp":1618523594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exploring the Potential Mechanism of Costunolide\u2010Induced MCF\u20107 Cells Apoptosis by Multi\u2010Spectroscopy, Molecular Docking and Cell Experiments"],"prefix":"10.1002","volume":"18","author":[{"given":"Dan","family":"Liu","sequence":"first","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Tianjin Ecological Academy of Environmental Sciences  17 Fukang Road Nankai District Tianjin Tianjin 300191 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Wen","family":"Pi","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei\u2010Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Zhe","family":"Ding","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Ying","family":"Mei","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Li","family":"Liu","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-7341","authenticated-orcid":false,"given":"Xiang\u2010Yu","family":"Cao","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,14]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2018.06.054"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2013.03.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.11.042"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.02.062"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21363"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2014.02.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2017.04.115"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2016.09.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2019.102679"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2017.01.003"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2019.07.099"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112256"},{"key":"e_1_2_7_13_1","first-page":"110952","article-title":"Synergistic anti-colon cancer effect of glycyrol and butyrate is associated with the enhanced activation of caspase-3 and structural features of glycyrol\u2019","volume":"8","author":"Lu S. Y.","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2017.06.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2014.04.028"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.12.075"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2019.100492"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/10731199.2010.494578"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000471234"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.10.082"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109142"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.explore.2016.04.005"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2018.1497543"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2018.09.012"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.03.022"},{"key":"e_1_2_7_26_1","first-page":"123","article-title":"Costunolide induces apoptosis in platinum-resistant human ovarian cancer cells by generating reactive oxygen species\u2019","author":"Yang Y. I.","year":"2011","journal-title":"Gynecol. Oncol."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2003.10.008"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.02.019"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.03.031"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2006.03.004"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.08.048"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2015.03.036"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2011.08.003"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.09.065"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2015.08.025"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2019.117525"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.11.061"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2007.03.008"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2014.09.056"},{"key":"e_1_2_7_40_1","first-page":"127595","article-title":"Novel fluorescence palladium-alkoxime complexes: Synthesis, characterization, DNA\/BSA spectroscopic and docking studies, evaluation of cytotoxicity and DNA cleavage mechanism\u2019","volume":"27","author":"Karami K.","year":"2019","journal-title":"J. Mol. Struct."},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2008.07.008"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2013.08.005"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2015.05.001"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2014.03.043"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2019.112067"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3MB70340A"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.29219\/fnr.v62.1323"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.01.011"},{"key":"e_1_2_7_49_1","first-page":"1847","article-title":"Alantolactone induces apoptosis and suppresses migration in MCF-7 human breast cancer cells via the p38 MAPK, NF-\u03baB and Nrf2 signaling pathways\u2019","volume":"42","author":"Liu J. L.","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2017.11.050"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.05.016"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.01.007"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2017.05.024"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2015.01.002"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2019.10.015"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2656"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.546"},{"key":"e_1_2_7_58_1","first-page":"5057","article-title":"Elevated cleaved caspase-3 is associated with shortened overall in survival cancer types\u2019","volume":"7","author":"Hu Q.","year":"2014","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2073-8"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1159\/000488433"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45039"},{"key":"e_1_2_7_62_1","first-page":"53","article-title":"Schizandra chinensis extracts induce apoptosis in human gastric cancer cells via JNK\/p38 MAPK activation and the ROS-mediated\/mitochondria-dependent pathway\u2019","author":"Kim H. S.","year":"2015","journal-title":"Pharm. Biol."},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.414-429.2002"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.08.065"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.06.024"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.03.053"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2018.06.057"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.05.033"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202001069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202001069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202001069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T00:46:24Z","timestamp":1693615584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202001069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,14]]},"references-count":68,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/cbdv.202001069"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202001069","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,14]]},"assertion":[{"value":"2020-12-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2001069"}}