{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:59:42Z","timestamp":1790841582778,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T00:00:00Z","timestamp":1622419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The production of self\u2010pollinated plants could be important for improving medicinal plants secondary metabolites. In this study, 11 <jats:italic>Thymus<\/jats:italic> populations from eight species were evaluated to determine the effect of self and open pollination on agro\u2010morphological characteristics, total phenolic content (TPC), essential oil (EO) content, and EO components. Inbreeding led to some positive effects of above mentioned traits in most of the studied populations. Total phenolic content ranged from 7.07 to 52.69\u2005mg tannic acid equivalents (TAE)\u2005g<jats:sup>\u22121<\/jats:sup> dry weight (DW) in open pollinated derived populations, while it varied from 1.2 to 55.03\u2005mg TAE\u2005g<jats:sup>\u22121<\/jats:sup> DW in self\u2010pollinated ones. Under open and self\u2010pollination condition, the highest EO content was obtained in <jats:italic>T. trautvetteri<\/jats:italic> (3.37\u2009%) and <jats:italic>T. pubescens<\/jats:italic> (1.96\u2009%), respectively. Gas chromatography\u2010mass spectrometry (GC\/MS) identified 42 compounds including thymol, carvacrol, linalool, <jats:italic>p\u2010<\/jats:italic>cymene, \u03b3\u2010terpinene, terpinen\u20104\u2010ol, and \u03b1\u2010terpineol as the main compounds. In most cases, selfed plants compared to open pollinated ones, revealed higher thymol content. <jats:italic>T. daenensis\u2010<\/jats:italic>1 showed a significant increase in thymol content (from 25.22\u2009% to 74.3\u2009%) due to self\u2010pollination. Moreover, self\u2010pollination led to emergence of some new compounds. Carvacrol methyl ether was the constituents of <jats:italic>Thymus<\/jats:italic> EO that are being reported in self\u2010pollinated populations. Finally, inbreeding in <jats:italic>Thymus<\/jats:italic> might be suggested as a useful tool to increase genetic homogeneity for the selection of superior plants for improving secondary metabolite.<\/jats:p>","DOI":"10.1002\/cbdv.202100097","type":"journal-article","created":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T18:37:55Z","timestamp":1619894275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Variation of Essential Oil Content and Composition, Phenolics, and Yield Related Traits Using Different Pollination Systems in Populations of <i>Thymus<\/i> Species"],"prefix":"10.1002","volume":"18","author":[{"given":"Faranak","family":"Khanahmadi","sequence":"first","affiliation":[{"name":"Department of Agronomy and Plant Breeding College of Agriculture Isfahan University of Technology  Isfahan 84156 83111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5853-8362","authenticated-orcid":false,"given":"Mehdi","family":"Rahimmalek","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding College of Agriculture Isfahan University of Technology  Isfahan 84156 83111 Iran"},{"name":"Department of Horticulture College of Agriculture Isfahan University of Technology  Isfahan 84156 83111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad R.","family":"Sabzalian","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding College of Agriculture Isfahan University of Technology  Isfahan 84156 83111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnaz","family":"Tohidi","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding College of Agriculture Isfahan University of Technology  Isfahan 84156 83111 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10600-009-9282-5"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.02.038"},{"key":"e_1_2_6_3_1","first-page":"205","article-title":"Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from Eastern Morocco\u2019","volume":"11","author":"Imelouane B.","year":"2009","journal-title":"Int. J. Agric. Biol."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32849-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.07.052"},{"key":"e_1_2_6_6_1","first-page":"377","article-title":"Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties\u2019","volume":"2","author":"Kabera J. N.","year":"2014","journal-title":"J. Pharm. Pharmacol."},{"key":"e_1_2_6_7_1","first-page":"61","article-title":"Essential oil composition of thyme (Thymus zygioides Griseb. var. lycaonicus (Celak.) Ronniger) wild growing in Turkey\u2019","volume":"8","author":"Duman E.","year":"2020","journal-title":"Turk. J. Agric. For."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.02.083"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2018.1434642"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.09.203"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2012.06.003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765X.2003.01259.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.1433"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.04.004"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2019.03.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-009-9281-z"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10725-013-9860-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2009.06.001"},{"key":"e_1_2_6_19_1","first-page":"80","article-title":"Essential oil of Cannabis sativa L. strains\u2019","volume":"4","author":"Mediavilla V.","year":"1997","journal-title":"J. Ind. Hemp."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.12.018"},{"key":"e_1_2_6_21_1","article-title":"Variation in bioactive contents and anatomical characteristics of different fennel (Foeniculum vulgare Mill.) populations as affected by self-pollination\u2019","volume":"89","author":"Salami M.","year":"2016","journal-title":"J. Appl. Bot. Food Qual."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2012.06.012"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1998.0011183X003800060017x"},{"key":"e_1_2_6_24_1","first-page":"447","article-title":"Karyological and nuclear DNA content variation in some Iranian endemic Thymus species (Lamiaceae)\u2019","volume":"12","author":"Mahdavi S.","year":"2010","journal-title":"J. Agric. Sci."},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/0972060X.2014.981600"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.12.046"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jarmap.2017.07.005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2012.07.044"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125521"},{"key":"e_1_2_6_30_1","first-page":"16","article-title":"Factors influencing the yield and the quality of hemp (Cannabis sativa L.) essential oil\u2019","volume":"5","author":"Meier C.","year":"1998","journal-title":"J. Ind. Hemp."},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-010-9636-1"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcata.2006.05.064"},{"key":"e_1_2_6_33_1","unstructured":"K.\u2005H. Rechinger \u2018Flora Iranica\u2019 Akademische Druck-U. Verlagsanstalt Graz Austria p. 49\u201371."},{"key":"e_1_2_6_34_1","first-page":"85","article-title":"Influence of growth stage on essential oil content and major chemical constituents of Artemisia pallens Bess\u2019","volume":"4","author":"Hiremath C.","year":"2020","journal-title":"Trends Phytochem. Res."},{"key":"e_1_2_6_35_1","first-page":"1902","article-title":"Identification of essential oil components by gas chromatography\/quadrupole mass spectrometry\u2019","volume":"16","author":"Adams R. P.","year":"2007","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(13)60142-2"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100097","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202100097","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T20:03:45Z","timestamp":1693425825000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202100097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,31]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/cbdv.202100097"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202100097","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,31]]},"assertion":[{"value":"2021-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2100097"}}