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In this study, we focused on the effects of <jats:italic>Moringa oleifera<\/jats:italic> Lam. on diabetes, attempted to isolate compounds that regulate NAFLD. Compounds <jats:bold>1<\/jats:bold> and <jats:bold>2<\/jats:bold> were isolated from the ethyl acetate fraction of <jats:italic>M. oleifera<\/jats:italic>. Spectral data revealed that they were 1\u2010hydroxy\u20103\u2010phenylpropan\u20102\u2010yl benzoate (<jats:bold>1<\/jats:bold>) and benzyl benzylcarbamate (<jats:bold>2<\/jats:bold>), respectively. The three\u2010dimensional structure of compound <jats:bold>1<\/jats:bold> was determined by single crystal X\u2010ray structural analysis. Neither compound was toxic to HepG2 cells, and compound <jats:bold>1<\/jats:bold> was found to have a concentration\u2010dependent inhibitory effect on intracellular lipid accumulation induced by stimulation of linoleic acid (LA). As a result of measuring the effects of compound <jats:bold>1<\/jats:bold> on the intracellular lipid production\u2010related protein, it was found that compound <jats:bold>1<\/jats:bold> enhanced protein expression that promotes lipolysis. On the other hand, since the action of compound <jats:bold>1<\/jats:bold> was similar to that of PPAR\u03b1 agonists, it is deduced that compound <jats:bold>1<\/jats:bold> enhanced the activity of PPAR\u03b1 and further enhanced the expression of lipolytic proteins, which is related to the suppression of intracellular lipid accumulation. Furthermore, as the result of docking simulation, compound <jats:bold>1<\/jats:bold> had a higher binding affinity to the ligand binding site of PPAR\u03b1 than fenofibrate, which is a PPAR\u03b1 agonist, and thus compound <jats:bold>1<\/jats:bold> was considered to be promising as an agonist of PPAR\u03b1.<\/jats:p>","DOI":"10.1002\/cbdv.202100243","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T06:29:16Z","timestamp":1623738556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Compounds from <i>Moringa oleifera<\/i> Lam. on <i>in Vitro<\/i> Non\u2010Alcoholic Fatty Liver Disease (NAFLD) Model System"],"prefix":"10.1002","volume":"18","author":[{"given":"Motohiko","family":"Ukiya","sequence":"first","affiliation":[{"name":"Department of Applied Chemistry College of Science and Technology Nihon University  1-5-1 Kandasurugadai Chiyoda Tokyo 101-0062 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Motegi","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry College of Science and Technology Nihon University  1-5-1 Kandasurugadai Chiyoda Tokyo 101-0062 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering Graduate School of Science and Engineering Yamagata University  4-3-16 Johnan Yonezawa 992-8510 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Kimura","sequence":"additional","affiliation":[{"name":"School of Medical Technology Faculty of Health Science Gunma Paz University  1-7-1 Tonyamachi Takasaki Gunma 370-0006 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Satsu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Maebashi Institute of Technology  460-1 Kamisadorimachi Maebashi Gunma 371-0816 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamoru","family":"Koketsu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Science Faculty of Engineering Gifu University  1-1 Yanagido Gifu 501-1193 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Ninomiya","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Science Faculty of Engineering Gifu University  1-1 Yanagido Gifu 501-1193 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lwin Mon","family":"Myint","sequence":"additional","affiliation":[{"name":"FAME Pharmaceuticals Industry Co., Ltd  Mingyi Mahar Min Gaung Street Yangon Myamar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6871-3707","authenticated-orcid":false,"given":"Atsuyoshi","family":"Nishina","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry College of Science and Technology Nihon University  1-5-1 Kandasurugadai Chiyoda Tokyo 101-0062 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080845"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30342-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116940"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071638"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0907929"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0000000000001260"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40261-019-00785-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S146339"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13679-019-00345-1"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-2835-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2016.08.003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/768540"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11030494"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b18-00702"},{"key":"e_1_2_7_15_1","unstructured":"K.\u2005M. 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