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The synthesized Schiff bases (<jats:bold>S<\/jats:bold>) were reacted with formaldehyde and secondary amine such as 4\u2010piperidinecarboxyamide to afford novel heterocyclic bases. 3\u2010Substitued\u20104\u2010(4\u2010hydroxybenzylidenamino)\u20104,5\u2010dihydro\u20101<jats:italic>H<\/jats:italic>\u20101,2,4\u2010triazol\u20105\u2010ones (<jats:bold>T<\/jats:bold>) were treated with 4\u2010piperidinecarboxyamide in the presence of formaldehyde to synthesize eight new 1\u2010(4\u2010piperidinecarboxyamide\u20101\u2010yl<jats:bold>\u2010<\/jats:bold>methyl)\u20103\u2010substitued\u20104\u2010(4\u2010hydroxybenzylidenamino)\u20104,5\u2010dihydro\u20101<jats:italic>H<\/jats:italic>\u20101,2,4\u2010triazol\u20105\u2010ones (<jats:bold>M1\u20108<\/jats:bold>). The structure characterization of compounds was carried out using <jats:sup>1<\/jats:sup>H\u2010NMR, IR, HR\u2010MS, and <jats:sup>13<\/jats:sup>C\u2010NMR spectroscopic methods. The inhibitory properties of the newly synthesized compounds were calculated against the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and glutathione S\u2010transferase (GST) enzymes. Ki values were calculated in the range of 20.06\u00b13.11\u201336.86\u00b16.17\u2005\u03bcM for GST, 17.87\u00b12.91\u201330.53\u00b14.25\u2005\u03bcM for AChE, 9.08\u00b10.69\u201320.02\u00b12.88\u2005\u03bcM for BChE, respectively, Besides, IC<jats:sub>50<\/jats:sub> values were also calculated. Best binding scores of \u2010inhibitors against used enzymes were calculated as \u221212.095\u2005kcal\/mol, \u221212.775\u2005kcal\/mol, and \u22129.336\u2005kcal\/mol, respectively. While 5\u2010oxo\u2010triazole piperidine\u20104\u2010carboxamide moieties have a critical role in the inhibition of AChE and GST enzymes, hydroxy benzyl moiety is important for BChE enzyme inhibition.<\/jats:p>","DOI":"10.1002\/cbdv.202100433","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T14:51:01Z","timestamp":1633099861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Synthesis, Spectroscopic Analysis, and <i>in Vitro\/in Silico<\/i> Biological Studies of Novel Piperidine Derivatives Heterocyclic Schiff\u2010Mannich Base Compounds"],"prefix":"10.1002","volume":"18","author":[{"given":"Song\u00fcl","family":"Boy","sequence":"first","affiliation":[{"name":"Atat\u00fcrk Vocational College of Health Service Kafkas University  Kars 36100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abd\u00fclmelik","family":"Aras","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science and Arts I\u011fd\u0131r University  I\u011fd\u0131r 76100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikret","family":"T\u00fcrkan","sequence":"additional","affiliation":[{"name":"Health Services Vocational School I\u011fd\u0131r University  I\u011fd\u0131r 76000 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onur","family":"Aky\u0131ld\u0131r\u0131m","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Faculty of Engineering and Architecture Kafkas University  Kars 36100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat","family":"Beytur","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science and Letters Kafkas University  Kars 36100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halide","family":"Sedef Karaman","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Ataturk University  Erzurum 25240 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevda","family":"Manap","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science and Letters Kafkas University  Kars 36100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haydar","family":"Y\u00fcksek","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science and Letters Kafkas University  Kars 36100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,20]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01809"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/hc-2020-0118"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.202000409"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4067\/s0717-97072020000204929"},{"key":"e_1_2_7_5_1","first-page":"494","article-title":"\u2018Synthesis, characterization and antioxidant properties of new 3-alkyl (aryl)-4-(3-hydroxy-4-methoxybenzylidenamino)-4, 5-dihydro-1H-1,2,4-triazol-5-ones\u2019","volume":"9","author":"Bah\u00e7eci \u015e.","year":"2016","journal-title":"Rasayanam"},{"key":"e_1_2_7_6_1","first-page":"98","article-title":"\u2018In-vitro biological activity of some new 1, 2, 4-triazole derivatives with their potentiometric titrations\u2019","volume":"49","author":"Akta\u015f-Yoku\u015f \u00d6.","year":"2017","journal-title":"Bulg. 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