{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T20:08:26Z","timestamp":1782590906750,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017964","name":"Tianjin Municipal Health Commission","doi-asserted-by":"publisher","award":["2020006"],"award-info":[{"award-number":["2020006"]}],"id":[{"id":"10.13039\/100017964","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gut dysbiosis contributes to hepatic fibrosis. Emerging evidence revealed the major role of traditional Chinese medicine (TCM) in gut microbiota homeostasis. Here, we aimed to investigate the anti\u2010fibrotic activity and underlying mechanism of ganshuang granules (GS), particularly regarding gut microbiota homeostasis. CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic fibrosis models were allocated into 4 groups receiving normal saline (model), 1.0, 2.0, or 4.0\u2005g\/kg GS for 5\u2005weeks. As result, GS treatment alleviated liver injury in CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic fibrosis, presenting as decreases of the liver index, alanine aminotransferase, and aspartate transaminase. Histological staining and expression revealed that the enhanced oxidative stress, inflammatory and hepatic fibrosis in CCl<jats:sub>4<\/jats:sub>\u2010induced models were attenuated by GS. Immunohistochemical staining showed that tight junction\u2010associated proteins in intestinal mucosa were up\u2010regulated by GS. 16S rRNA sequencing showed that GS rebalanced the gut dysbiosis manifested as improving alpha and beta diversity of gut microbiota, reducing the ratio of Firmicutes to Bacteroidetes, and regulating the relative abundance of various bacteria. In summary, GS decreased the intestinal permeability and rebalanced the gut microbiota to reduce the oxidative stress and inflammation, eventually attenuating CCl<jats:sub>4<\/jats:sub>\u2010induced hepatic fibrosis.<\/jats:p>","DOI":"10.1002\/cbdv.202100520","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T15:44:28Z","timestamp":1632930268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Traditional Chinese Medicine Ganshuang Granules Attenuate CCl<sub>4<\/sub>\u2010Induced Hepatic Fibrosis by Modulating Gut Microbiota"],"prefix":"10.1002","volume":"18","author":[{"given":"Jie","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wei","sequence":"additional","affiliation":[{"name":"Graduate School Tianjin University of Traditional Chinese Medicine  No. 10, Poyanghu Road, Town West Area, Jinghai District Tianjin 301617 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Tianjin Second People's Hospital  No. 7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine Tianjin Second People's Hospital  No.7, Sudi Nan Road, Naikai District Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199306243282508"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0881-3.00005-X"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00261-017-1211-7"},{"key":"e_1_2_10_4_1","first-page":"27","article-title":"\u2018Hepatic fibrosis-role of hepatic stellate cell activation\u2019","volume":"4","author":"Safadi R.","year":"2002","journal-title":"Med. 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