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The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high\u2010performance liquid chromatography, and the antioxidant activities were assayed by DPPH<jats:sup>.<\/jats:sup> and ABTS<jats:sup>.+<\/jats:sup>. Minimal inhibitory and bactericidal concentrations (MIC\/MBC) were applied to twenty\u2010two bacteria. Most strains susceptible to extract\/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug\u2010resistant strains in modulatory and <jats:italic>checkboard<\/jats:italic> models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH<jats:sup>.<\/jats:sup> and ABTS<jats:sup>.+<\/jats:sup> assays. Gram\u2010positive strains are more susceptible to EAF. The subinhibitory concentrations of <jats:italic>P. granatum<\/jats:italic> enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics.<\/jats:p>","DOI":"10.1002\/cbdv.202100538","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T15:01:23Z","timestamp":1633618883000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of Antioxidant, Antibacterial and Enhancement of Antibiotic Action by <i>Punica granatum<\/i> Leaves Crude Extract and Enriched Fraction against Multidrug\u2010Resistant Bacteria"],"prefix":"10.1002","volume":"18","author":[{"given":"Liliane Bezerra","family":"de Lima","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Universidade Federal de Pernambuco  Av. da Engenharia, s\/n, Cidade Universit\u00e1ria 50740-600 Recife-PE 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