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The new structures were elucidated as (3<jats:italic>S<\/jats:italic>,14<jats:italic>R<\/jats:italic>)\u20103,14\u2010dihydroxycholesta\u20105,8\u2010dien\u20107\u2010one (<jats:bold>1<\/jats:bold>), (22<jats:italic>E<\/jats:italic>,24<jats:italic>R<\/jats:italic>)\u20107<jats:italic>\u03b1<\/jats:italic>\u2010ethoxy\u20105<jats:italic>\u03b1<\/jats:italic>,6<jats:italic>\u03b1<\/jats:italic>\u2010epoxyergosta\u20108(14),22\u2010dien\u20103<jats:italic>\u03b2<\/jats:italic>\u2010ol (<jats:bold>2<\/jats:bold>), 3<jats:italic>\u03b2<\/jats:italic>\u2010hydroxy\u20107<jats:italic>\u03b1<\/jats:italic>\u2010ethoxy\u20105<jats:italic>\u03b1<\/jats:italic>,6<jats:italic>\u03b1<\/jats:italic>\u2010epoxy\u20108(14)\u2010cholestene (<jats:bold>3<\/jats:bold>), 3<jats:italic>\u03b2<\/jats:italic>,5<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20106<jats:italic>\u03b1<\/jats:italic>\u2010ethoxychofesta\u20107,9(11)\u2010diene (<jats:bold>4<\/jats:bold>), 3<jats:italic>\u03b2<\/jats:italic>,5<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20106<jats:italic>\u03b2<\/jats:italic>\u2010ethoxycholest\u20107\u2010ene (<jats:bold>5<\/jats:bold>), (22<jats:italic>E<\/jats:italic>,24<jats:italic>R<\/jats:italic>)\u201024\u2010ethoxy\u20103<jats:italic>\u03b2<\/jats:italic>,5<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20106<jats:italic>\u03b2<\/jats:italic>\u2010ethoxyergosta\u20107,22\u2010diene (<jats:bold>6<\/jats:bold>), (22<jats:italic>E<\/jats:italic>)\u20103<jats:italic>\u03b2<\/jats:italic>,5<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20106<jats:italic>\u03b2<\/jats:italic>\u2010ethoxycholesta\u20107,22\u2010diene (<jats:bold>7<\/jats:bold>), 24\u2010ethoxy\u20103<jats:italic>\u03b2<\/jats:italic>,5<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20106<jats:italic>\u03b2<\/jats:italic>\u2010ethoxycholest\u20107\u2010ene (<jats:bold>8<\/jats:bold> and <jats:bold>9<\/jats:bold>), by extensive spectroscopic analyses, such as HR\u2010ESI\u2010MS, 1D and 2D NMR data. The absolute configuration of <jats:bold>1<\/jats:bold> was assigned by comparison the experimental ECD spectra with the calculated ones. Among the 13 metabolites, compounds <jats:bold>1<\/jats:bold>, <jats:bold>4<\/jats:bold>, <jats:bold>11<\/jats:bold>, <jats:bold>12<\/jats:bold>, and <jats:bold>13<\/jats:bold> showed NF\u2010\u03baB inhibitory activities in human HER\u2010293 cells with IC<jats:sub>50<\/jats:sub> values of 6.4, 18.7, 8.1, 9.6, and 7.5\u2005\u03bcM, respectively. Preliminary structure\u2212activity relationship analysis unveiled that the conjugated ketones or unsaturated double bonds might be the functional groups for the five active steroids.<\/jats:p>","DOI":"10.1002\/cbdv.202100578","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T17:01:07Z","timestamp":1631206867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["New NF\u2010\u03baB Inhibitory Steroids from the Marine Sponge <i>Dysidea avara<\/i> Collected from the South China Sea"],"prefix":"10.1002","volume":"18","author":[{"given":"Lu","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University  Shenyang 110016 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Ling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University  Shenyang 110016 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian\u2010Xian","family":"Miao","sequence":"additional","affiliation":[{"name":"Research Center for Marine Drugs, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Marine Drugs, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hou\u2010Wen","family":"Lin","sequence":"additional","affiliation":[{"name":"Research Center for Marine Drugs, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Hua","family":"Jiao","sequence":"additional","affiliation":[{"name":"Research Center for Marine Drugs, State Key Laboratory of Oncogenes and Related Genes, Department of Pharmacy, Ren Ji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200127 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_1_1","DOI":"10.1016\/S0040-4039(01)91918-5"},{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1021\/jo00176a005"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1021\/jo00154a029"},{"key":"e_1_2_7_4_1","first-page":"4771","article-title":"\u2018Novel Cytotoxic Polyoxygenated Steroids from an Okinawan Sponge Dysidea sp.\u2019","volume":"50","author":"Govindam S. 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