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Cardamonin, a natural chalcone with <jats:italic>in\u2005vitro<\/jats:italic> schistosomicidal activity, has not been <jats:italic>in\u2005vivo<\/jats:italic> evaluated against <jats:italic>Schistosoma<\/jats:italic>. In this work, we evaluated the <jats:italic>in\u2005vivo<\/jats:italic> schistosomicidal activities of cardamonin against <jats:italic>Schistosoma mansoni<\/jats:italic> worms and conducted enzymatic apyrase inhibition assay, as well as molecular docking analysis of cardamonin against potato apyrase, <jats:italic>S.\u2005mansoni<\/jats:italic> NTPDase\u20051 and <jats:italic>S.\u2005mansoni<\/jats:italic> NTPDase\u20052. In a mouse model of schistosomiasis, the oral treatment with cardamonin (400\u2005mg\/kg) showed efficacy against <jats:italic>S.\u2005mansoni<\/jats:italic>, decreasing the total worm load in 46.8\u2009% and reducing in 54.5\u2009% the number of eggs in mice. Cardamonin achieved a significant inhibition of the apyrase activity and the three\u2010dimensional structure of the potato apyrase, obtained by homology modeling, showed that cardamonin may interact mainly through hydrogen bonds. Molecular docking studies corroborate with the action of cardamonin in binding and inhibiting both potato apyrase and <jats:italic>S.\u2005mansoni<\/jats:italic> NTPDases.<\/jats:p>","DOI":"10.1002\/cbdv.202100604","type":"journal-article","created":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T06:51:47Z","timestamp":1633503107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Cardamonin Presents <i>in Vivo<\/i> Activity against <i>Schistosoma mansoni<\/i> and Inhibits Potato Apyrase"],"prefix":"10.1002","volume":"18","author":[{"given":"Lara Soares Aleixo","family":"de Carvalho","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Department of Pharmaceutical Sciences Federal University of Juiz de Fora  Juiz de Fora MG, 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia Mara","family":"Silva","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Department of Pharmaceutical Sciences Federal University of Juiz de Fora  Juiz de Fora MG, 36036-900 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Carius","family":"de Souza","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Modelagem Computacional Departamento de Ci\u00eancia da Computa\u00e7\u00e3o, ICE Universidade Federal de Juiz de Fora  Juiz de Fora MG, 36036-900 Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Paulo Nascimento","family":"da Silva","sequence":"additional","affiliation":[{"name":"Research Center for Neglected Diseases Guarulhos University  Guarulhos SP, 07025-000 Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9780-4328","authenticated-orcid":false,"given":"Priscila V. 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