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DFT calculations have revealed a distorted octahedral for <jats:bold>1<\/jats:bold>, <jats:bold>2<\/jats:bold> and <jats:bold>3<\/jats:bold>. DNA interactions of <jats:bold>1<\/jats:bold>\u2013<jats:bold>3<\/jats:bold> toward calf\u2010thymus DNA were investigated by absorption titration, viscosity, fluorescence spectroscopy and gel electrophoresis. All complexes bind to DNA <jats:italic>via<\/jats:italic> intercalation mode. The <jats:italic>K<\/jats:italic><jats:sub>b<\/jats:sub> values have shown the binding ability in the order of <jats:bold>3<\/jats:bold>&gt;<jats:bold>2<\/jats:bold>&gt;<jats:bold>1<\/jats:bold>. The <jats:italic>in\u2005vitro<\/jats:italic> cytotoxicity of <jats:bold>1<\/jats:bold>\u2013<jats:bold>3<\/jats:bold> was tested against two human cancer cells (HeLa and MCF\u20107) and normal Vero cells by MTT assay. All complexes show anticancer ability upon increasing time, but the ligand is inactive. Complex <jats:bold>3<\/jats:bold> with the Br<jats:sup>\u2212<\/jats:sup> anion gives the lowest IC<jats:sub>50<\/jats:sub> values of 19.26\u00b12.43\u2005\u03bcg\/mL toward MCF\u20107. Moreover, all complexes were not arrested at any cell cycle phases. However, the sub\u2010G1 populations were enhanced upon increasing the complex concentration. Therefore, they may induce apoptotic cell death for both cancer cells. The antibacterial activity of <jats:bold>1<\/jats:bold>\u2013<jats:bold>3<\/jats:bold> was tested against <jats:italic>E.\u2005coli<\/jats:italic>, <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Campylobacter<\/jats:italic>. Complex <jats:bold>3<\/jats:bold> gives the best activity toward <jats:italic>Campylobacter<\/jats:italic> (0.156\u00d710<jats:sup>3<\/jats:sup>\u2005\u03bcg\/mL).<\/jats:p>","DOI":"10.1002\/cbdv.202100708","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T03:58:18Z","timestamp":1642737498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Copper(II) Compounds of 4\u2010Nitrobenzohydrazide with Different Anions (ClO<sub>4<\/sub><sup>\u2212<\/sup>, NO<sub>3<\/sub><sup>\u2212<\/sup> and Br<sup>\u2212<\/sup>): Synthesis, Characterization, DFT Calculations, DNA Interactions and Cytotoxic Properties"],"prefix":"10.1002","volume":"19","author":[{"given":"Prangtip","family":"Nonkuntod","sequence":"first","affiliation":[{"name":"Materials Chemistry Research Center Department of Chemistry and Center of Excellence for Innovation in Chemistry Faculty of Science Khon Kaen University  Khon Kaen 40002 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanaset","family":"Senawong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science Khon Kaen University  Khon Kaen 40002 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaiyaporn","family":"Soikum","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health Faculty of Veterinary Medicine Khon Kaen University  Khon Kaen 40002 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prapansak","family":"Chaveerach","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health Faculty of Veterinary Medicine Khon Kaen University  Khon Kaen 40002 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athis","family":"Watwiangkham","sequence":"additional","affiliation":[{"name":"School of Chemistry Institute of Science Suranaree University of Technology  Nakhon Ratchasima 30000 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suwit","family":"Suthirakun","sequence":"additional","affiliation":[{"name":"School of Chemistry Institute of Science Suranaree University of Technology  Nakhon Ratchasima 30000 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1623-2394","authenticated-orcid":false,"given":"Unchulee","family":"Chaveerach","sequence":"additional","affiliation":[{"name":"Materials Chemistry Research Center Department of Chemistry and Center of Excellence for Innovation in Chemistry Faculty of Science Khon Kaen University  Khon Kaen 40002 Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,15]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/205698a0"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/222385a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(84)90049-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00175a020"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200363150-00004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(90)90065-S"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/12081910"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20021936"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm049913k"},{"key":"e_1_2_8_10_1","first-page":"290","article-title":"\u2018Complexes of platinum and palladium with 4-nitrobenzohydrazide: Synthesis and cytotoxic activity\u2019","volume":"11","author":"Souza G. 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