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The toxic effect of MGO (0.5, 1, and 2\u2005mM) on human embryonic kidney (HEK293) cells and its underlying mechanisms were explored in this study. Cell viability, apoptosis and the signaling pathways were measured with MTT, fluorescent staining and western blot experiments, the results showed that MGO could induce oxidative stress and cell inflammation, the level of reactive oxygen species (ROS) increased, and p38MAPK, JNK and NF\u2010\u03baB signaling pathways were activated. Meanwhile, MGO also induced DNA damage. The expression of DNA oxidative damage marker 8\u2010hydroxy\u20102\u2019\u2010deoxyguanosine (8\u2010OHdG) increased, the expression of double\u2010strand break marker \u03b3H2AX increased significantly, and ATM\/Chk2\/p53 DNA damage response signaling pathway was activated. Furthermore, the expression of the receptor for advanced glycation end products (RAGE) also increased. Finally, mitochondrial membrane potential (MMP) decreased, fluorescence intensity of Hoechst33258 increased, and the protein expression ratio of Bax\/Bcl\u20102 increased significantly after the treatment of MGO. These results demonstrated that MGO might induce HEK293 cells damage by regulating oxidative stress, inflammation, DNA damage, and cell apoptosis, which revealed the specific mechanisms of MGO\u2010induced damage to HEK293 cells.<\/jats:p>","DOI":"10.1002\/cbdv.202100829","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T04:08:59Z","timestamp":1640664539000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Potential Mechanisms of Methylglyoxal\u2010Induced Human Embryonic Kidney Cells Damage: Regulation of Oxidative Stress, DNA Damage, and Apoptosis"],"prefix":"10.1002","volume":"19","author":[{"given":"Dan","family":"Liu","sequence":"first","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Tang","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junliang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xia","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Environment Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5847-7341","authenticated-orcid":false,"given":"Xiangyu","family":"Cao","sequence":"additional","affiliation":[{"name":"School of life Science Liaoning University  66 Chongshan Middle Road Shenyang 110036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119635"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105192"},{"key":"e_1_2_7_3_1","unstructured":"IDF Diabetes Atlas Diabetes around the world in 2021.https:\/\/diabetesatlas.org\/. 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