{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:31:44Z","timestamp":1767918704292,"version":"3.49.0"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Essential oils (EOs) of <jats:italic>Salvia fruticosa<\/jats:italic> Mill. gathered from three Palestinian localities were studied to determine their constituents, antibacterial adhesion impact against <jats:italic>Klebsiella pneumonia<\/jats:italic>, cytotoxicity, and their function in cancer cell migration. Gas chromatography\u2010mass spectrometry identified the chemical components, while the MTT technique was used to measure the EOs\u2032 cytotoxicity against HeLa (cervical) and Caco\u20102 (colorectal) cancer cells. Antibacterial adhesion was assessed by examining <jats:italic>Klebsiella pneumoniae<\/jats:italic>\u2019s ability to adhere to Caco\u20102 cells. Our study found that eucalyptol was present as the main constituent in all <jats:italic>S. fruticosa<\/jats:italic> EOs. In addition, <jats:italic>K. pneumoniae<\/jats:italic> adhesion and metastasis were reduced after 48 h of application. Salfit's and Hebron's EOs had the most potent cytotoxic effects on Caco\u20102 and HeLa, with IC<jats:sub>50<\/jats:sub> values in the range of 0.7\u20131.3 \u03bcg\/mL. Taking into account the obtained results, it may be concluded that <jats:italic>S. fruticosa<\/jats:italic> EOs can serve as potential disinfectant agents for the treatment and prevention of <jats:italic>K. pneumoniae\u2010<\/jats:italic>associated illness and cancer.<\/jats:p>","DOI":"10.1002\/cbdv.202100872","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T13:43:02Z","timestamp":1644932582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Constituents, Antibacterial Adhesion, Cytotoxic and <i>in Vitro<\/i> Metastasis Blocking Properties of <i>Salvia fruticosa<\/i> Essential Oils from Three Palestinian Localities"],"prefix":"10.1002","volume":"19","author":[{"given":"Nidal","family":"Jaradat","sequence":"first","affiliation":[{"name":"Department of Pharmacy Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samer","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestine"},{"name":"Department of Biology &amp; Biotechnology Faculty of Science An-Najah National University  Nablus P.O. Box 7 State of Palestine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nawaf","family":"Al\u2010Maharik","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science An-Najah National University  Nablus P.O. Box. 7 State of Palestine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2291-6821","authenticated-orcid":false,"given":"Mohammad","family":"Altamimi","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Technology An-Najah National University  Nablus P.O. Box 7 State of Palestine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Hawash","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Qneibi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer","family":"Abu Khair","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anaal","family":"Zetawi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leena","family":"Jabarin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Medicine and Health Sciences An-Najah National University  Nablus P.O. Box 7 State of Palestin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2021.01.008"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061578"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"\u2018An overview of the genus Aloysia Pal\u00e1u (Verbenaceae): Essential oil composition, ethnobotany and biological activities\u2019","volume":"2021","author":"Mohammadhosseini M.","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26164766"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26154664"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12992-014-0081-9"},{"key":"e_1_2_8_7_1","unstructured":"World Health Organization. NCDs and development Geneva: WHO; 2016 [Available from:https:\/\/www.who.int\/nmh\/publications\/ncd\u2002report\u2002chapter2.pdf."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31992-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","unstructured":"K. Atkinson D. Mabey \u2018The Burden of Communicable Diseases in Low- and Middle-Income Countries\u2019 Willy online library USA 2019.","DOI":"10.1002\/9781119282686.ch1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2547-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00064-12"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2015.09.036"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.018"},{"key":"e_1_2_8_14_1","first-page":"1","article-title":"\u2018Primary Klebsiella pneumoniae Osteomyelitis with Bacteremia and Sepsis in a Patient with Cirrhosis\u2019","volume":"18","author":"Khatri A.","year":"2018","journal-title":"Case Rep. Infect. Dis."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2017.05.009"},{"key":"e_1_2_8_16_1","first-page":"1","article-title":"\u2018The genus Perovskia Kar.: ethnobotany, chemotaxonomy and phytochemistry: a review\u2019","volume":"40","author":"Mohammadhosseini M.","year":"2019","journal-title":"Toxin Rev."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/1385272823666191007161550"},{"key":"e_1_2_8_18_1","first-page":"89","article-title":"\u2018Characterization of essential oils of some Salvia species and their antimycobacterial activities\u2019","volume":"34","author":"A\u015fkun T.","year":"2010","journal-title":"Turk. J. Biol."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)84802-5"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2547169"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.02.013"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf9708296"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0211534"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(91)90030-H"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","unstructured":"L. Pizzale R. Bortolomeazzi S. Vichi E. \u00dcberegger L.\u2005S. Conte \u2018Antioxidant activity of sage (Salvia officinalisandS fruticosa) and oregano (Origanum onitesandO. indercedens) extracts related to their phenolic compound content\u2019 J. Sci. Food Agric.2002 82 1645\u201351.","DOI":"10.1002\/jsfa.1240"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.2298\/ABS1301321G"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-009-9122-z"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7178105"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)61333-5"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635580802710733"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1865-3"},{"key":"e_1_2_8_32_1","first-page":"745","article-title":"\u2018Polyphenolic profile and biological study of Salvia fruticosa\u2019","volume":"1","author":"Moharram F. A.","year":"2006","journal-title":"Nat. Prod. Commun."},{"key":"e_1_2_8_33_1","doi-asserted-by":"crossref","unstructured":"G. Omar M. Dwikat L. Abdallah S. Ismaeil \u2018Effect of Ethanol Extract from Five Species of Salvia on the Spontaneous Contractile Activity of Isolated Rabbit Ileum\u2019 Palestinian Med. Pharm. J. 4 40\u20131.","DOI":"10.59049\/2790-0231.1032"},{"key":"e_1_2_8_34_1","unstructured":"R. Harris \u2018Handbook of Essential Oils: Science Technology and Applications\u2019 Taylor & Francis Group United Kingdom 2015."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf800802t"},{"key":"e_1_2_8_36_1","first-page":"1","article-title":"\u2018Quality control of the traditional herbs and herbal products: a review\u2019","volume":"6","author":"Balekundri A.","year":"2020","journal-title":"Future J. Pharm. Sci."},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27020349"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10030574"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/w12020380"},{"key":"e_1_2_8_40_1","first-page":"619","article-title":"\u2018Terpenoids, essential oil composition, fatty acid profile, and biological activities of Anatolian Salvia fruticosa Mill\u2019","volume":"37","author":"Top\u00e7u G.","year":"2013","journal-title":"Turk. J. Chem."},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.33320\/maced.pharm.bull.2015.61.01.004"},{"key":"e_1_2_8_42_1","first-page":"25","article-title":"\u2018Essential oils\u2019 chemical characterization and investigation of some biological activities: a critical review\u2019","volume":"3","author":"Dhifi W.","year":"2016","journal-title":"Medicine"},{"key":"e_1_2_8_43_1","unstructured":"L. Mander H.-W. Liu \u2018Comprehensive natural products II: Chemistry and Biology\u2019 Elsevier UK 2010."},{"key":"e_1_2_8_44_1","first-page":"125","article-title":"\u2018Eucalyptol: safety and pharmacological profile\u2019","volume":"5","author":"Bhowal M.","year":"2015","journal-title":"J. Pharm. Sci."},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1573(200006)14:4<240::AID-PTR573>3.0.CO;2-X"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1139\/y02-142"},{"key":"e_1_2_8_47_1","first-page":"67","article-title":"\u2018Therapy with expectorants: a double-blind randomised study comparing ambroxol and cineol\u2019","volume":"24","author":"Wittmann M.","year":"1998","journal-title":"Atemw-Lungenkrkh"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2763"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748233710388452"},{"key":"e_1_2_8_50_1","first-page":"307","article-title":"\u2018Antibacterial activity of halothane, eucalyptol and orange oil\u2019","volume":"34","author":"Karlovi\u0107 Z.","year":"2000","journal-title":"Acta Stomatol. Croat."},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1211\/0022357011775604"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2009.11.014"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00078-15"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdsr.2021.05.003"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.71786"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf970031m"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880200490902590"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-01161-4"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2012.00875.x"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/859083"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.4172\/2167-7956.1000154"},{"key":"e_1_2_8_62_1","first-page":"1","article-title":"\u2018Quantitative estimations for the volatile Oil by using hydrodistillation and microwave accelerated distillation methods from Ruta graveolens L. and Ruta chalepensis L. leaves from Jerusalem area\/Palestine\u2019","volume":"4","author":"Jaradat N.","year":"2016","journal-title":"Mor. J. Chem."},{"key":"e_1_2_8_63_1","unstructured":"National Institute of Standards and Technology NIST\/EPA\/NIH Mass Spectral Library \u2018The NIST Mass Spectrometry Data Center\u2019 NIST Gaithersburg MD USA 2014."},{"key":"e_1_2_8_64_1","unstructured":"R.\u2005P. Adams \u2018Identification of essential oil components by gas chromatography\/mass spectrometry\u2019 Allured Publishing Corporation Carol Stream IL USA 2007."},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2016.03.010"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjp.2019.06.004"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100872","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202100872","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202100872","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T22:50:02Z","timestamp":1693003802000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202100872"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,28]]},"references-count":66,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/cbdv.202100872"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202100872","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,28]]},"assertion":[{"value":"2021-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202100872"}}