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In this study, the growth suppressive effect of Zn(II) 5,5\u2010diethylbarbiturate complex on MCF\u20107 and MDA\u2010MB\u2010231 human breast cancer cells was determined by SRB and ATP viability assays and apoptosis\u2010inducing effect by double staining method. Significant increase in cytokeratin 18 level, caspase 3\/7 activity and annexin\u2010V upregulation prove that Zn(II) complex has apoptotic effect in breast cancer cells. Intrinsic apoptosis pathway in MCF\u20107 cells and extrinsic apoptosis pathway in MDA\u2010MB\u2010231 cells was determined by Western blot (PARP, Cleave PARP, BAX, COX4, RIP, Caspase 8, Split Caspase 8, DR4 and B\u2010Actin) and RT\u2010PCR (PARP, Fas, Bcl\u20102, TNF10A, P53) analysis. No reduction of viability was found in MCF\u2010710A healthy breast cells treated with Zn(II) complex. In breast cancer stem\u2010like cells (MCF\u20107s), the Zn(II) complex was found to have a cytotoxic effect and to activate the apoptotic pathway. As a result, it was concluded that Zn(II) complex has anti\u2010proliferative and apoptotic effects on breast cancer and breast cancer stem\u2010like cells. Also this complex prevents the metastatic effect of cancer cells and does not effect to healthy cells so this complex has a specific effect on cancer cells. These findings might shed light on the discovery of new chemotherapeutic agents.<\/jats:p>","DOI":"10.1002\/cbdv.202101001","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T13:38:35Z","timestamp":1646660315000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Zn(II) 5,5\u2010Diethylbarbiturate Complex Selectively Induces Apoptosis in Breast Cancer and Breast Cancer Stem\u2010Like Cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Imren","family":"Alioglu","sequence":"first","affiliation":[{"name":"Department of Biology Bursa Uludag University Science and Art Faculty  16059 Bursa Turkey"},{"name":"Department of Molecular Biology and Genetics Democritus University of Thrace  68100 Alexandroupolis Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilias","family":"Tsochantaridis","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Democritus University of Thrace  68100 Alexandroupolis Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aglaia","family":"Pappa","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Democritus University of Thrace  68100 Alexandroupolis Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egemen","family":"Dere","sequence":"additional","affiliation":[{"name":"Department of Biology Bursa Uludag University Science and Art Faculty  16059 Bursa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferda","family":"Ari","sequence":"additional","affiliation":[{"name":"Department of Biology Bursa Uludag University Science and Art Faculty  16059 Bursa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,21]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0530291100"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1188\/05.ONF.605-616"},{"key":"e_1_2_8_3_1","first-page":"1","article-title":"\u2018Palladium-based chemotherapeutic agents: Routes toward complexes with good antitumor activity\u2019","volume":"6","author":"Salim Abu-Surrah A.","year":"2008","journal-title":"Cancer Ther."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1529"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1534735404270335"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.poly.2015.09.057"},{"key":"e_1_2_8_7_1","first-page":"1529","article-title":"\u2018Metals and Metal Compounds in Cancer Treatment\u2019","volume":"24","author":"Desoize B.","year":"2004","journal-title":"Anticancer Res."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.11.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2867-13-62"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/b913236h"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161210791209009"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9mt00124g"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.12971"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo_32_6_1189"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2018.05.002"},{"key":"e_1_2_8_16_1","first-page":"77","article-title":"\u2018Breast Cancer Stem Cells\u2019","volume":"6","author":"Crabtree J. 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