{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:11:53Z","timestamp":1790928713629,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The root extract of <jats:italic>Nauclea xanthoxylon<\/jats:italic> (A.Chev.) Aubr\u00e9v. displayed significant 50\u2009% inhibition concentration (IC<jats:sub>50<\/jats:sub>s) of 0.57 and 1.26\u2005\u03bcg\/mL against chloroquine resistant and sensitive <jats:italic>Plasmodium falciparum<\/jats:italic> (<jats:italic>Pf<\/jats:italic>) Dd2 and 3D7 strains, respectively. Bio\u2010guided fractionation led to an ethyl acetate fraction with IC<jats:sub>50<\/jats:sub>s of 2.68 and 1.85\u2005\u03bcg\/mL and subsequently, to the new quinovic acid saponin named xanthoxyloside (<jats:bold>1<\/jats:bold>) with IC<jats:sub>50<\/jats:sub>s of 0.33 and 1.30\u2005\u03bcM, respectively against the tested strains. Further compounds obtained from ethyl acetate and hexane fractions were the known clethric acid (<jats:bold>2<\/jats:bold>), ursolic acid (<jats:bold>3<\/jats:bold>), quafrinoic acid (<jats:bold>4<\/jats:bold>), quinovic acid (<jats:bold>5<\/jats:bold>), quinovic acid 3\u2010<jats:italic>O<\/jats:italic>\u2010<jats:italic>\u03b2<\/jats:italic>\u2010D\u2010fucopyranoside (<jats:bold>6<\/jats:bold>), oleanolic acid (<jats:bold>7<\/jats:bold>), oleanolic acid 3\u2010acetate (<jats:bold>8<\/jats:bold>), friedelin (<jats:bold>9<\/jats:bold>), \u03b2\u2010sitosterol (<jats:bold>10a<\/jats:bold>), stigmasterol (<jats:bold>10b<\/jats:bold>) and stigmasterol 3\u2010<jats:italic>O\u2010\u03b2\u2010<\/jats:italic>D\u2010glucopyranoside (<jats:bold>11<\/jats:bold>). Their structures were characterised with the aid of comprehensive spectroscopic methods (1 and 2D NMR, Mass). Bio\u2010assays were performed using nucleic acid gel stain (SYBR green I)\u2010based fluorescence assay with chloroquine as reference. Extracts and compounds exhibited good selectivity indices (SIs) of &gt;10. Significant antiplasmodial activities measured for the crude extract, the ethyl acetate fraction and xanthoxyloside (<jats:bold>1<\/jats:bold>) from that fraction can justify the use of the root of <jats:italic>N. xanthoxylon<\/jats:italic> in ethnomedicine to treat malaria.<\/jats:p>","DOI":"10.1002\/cbdv.202200271","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T03:21:47Z","timestamp":1678332107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bioguided Fractionation and Isolation of an Antiplasmodial Saponin from the Roots of <i>Nauclea xanthoxylon<\/i> (A.Chev.) Aubr\u00e9v. (Rubiaceae)"],"prefix":"10.1002","volume":"20","author":[{"given":"Brice Maxime","family":"Nkouayeb Nangmou","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science University of Douala  P.O. Box 24157 Douala Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermine Laure","family":"Maza Djomkam","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science University of Douala  P.O. Box 24157 Douala Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Bellier Tabekoueng","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science University of Douala  P.O. Box 24157 Douala Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willifred Dongmo","family":"T\u00e9kapi Tsopgni","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science University of Douala  P.O. Box 24157 Douala Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabin Thierry","family":"Mbahbou Bitchagno","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry Faculty of Science University of Dschang P.O. Box 67  Dschang Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel Arnaud","family":"Mbock","sequence":"additional","affiliation":[{"name":"Department of Biohemistry Faculty of Science University of Yaound\u00e9 I  P.O. Box 812 Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raceline Gounoue","family":"Kamkumo","sequence":"additional","affiliation":[{"name":"Department of Biohemistry Faculty of Science University of Yaound\u00e9 I  P.O. Box 812 Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Frese","sequence":"additional","affiliation":[{"name":"Organic and Bioorganic Chemistry Faculty of Chemistry Bielefeld University  D-33501 Bielefeld Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Ndjakou Lenta","sequence":"additional","affiliation":[{"name":"Department of Chemistry Higher Teacher Training College University of Yaound\u00e9 I  P.O. Box 47 Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silv\u00e8re Augustin","family":"Ngouela","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry Faculty of Science University of Dschang P.O. 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