{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:02:15Z","timestamp":1791910935103,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypertrophic cardiomyopathy is a major cause of mortality worldwide. In this study, we hypothesized that the combination of Dantrolene and Polymyxin\u2010B will provide cardioprotective action against isoproterenol\u2010induced hypertrophic cardiomyopathy via attenuation of Calmodulin\u2010dependent protein kinase II (CaMKII). Hypertrophic cardiomyopathy was induced in rats by subcutaneous administration of isoproterenol (5\u2005mg\/kg) for 14\u2005days. Simultaneously, animals were treated with Polymyxin\u2010B <jats:italic>per se<\/jats:italic>, Dantrolene <jats:italic>per se<\/jats:italic>, and Dantrolene and Polymyxin\u2010B combination for 14\u2005days. Hemodynamic parameters, biochemical parameters, and histological analysis were performed. Administration of isoproterenol for 14\u2005days resulted in severe myocardial damage, characterized by cardiac hypertrophy and increase serum CK\u2010MB, CK\u2010Nac, LDH, AST, and ALT levels. It also caused alteration in electrocardiogram and blood pressure. A significant increase in CaMKII was observed in heart homogenate. Treatment with the Polymyxin\u2010B and Dantrolene combination significantly ameliorated cardiac hypertrophy, biochemical parameters, ECG parameters, and heart histopathology. Further, significant attenuation in CaMKII levels was observed. The effect of the combination was more than <jats:italic>per se<\/jats:italic> treatment. Results of the current study showed that the combination of Polymyxin\u2010B and Dantrolene prevented the development of isoproterenol\u2010induced hypertrophic cardiomyopathy in rats via attenuation of the CaMKII.<\/jats:p>","DOI":"10.1002\/cbdv.202200309","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T17:38:17Z","timestamp":1661794697000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cardioprotective Effect of Polymyxin\u2010B and Dantrolene Combination on Isoproterenol\u2010Induced Hypertrophic Cardiomyopathy in Rats, via Attenuation of Calmodulin\u2010Dependent Protein Kinase II"],"prefix":"10.1002","volume":"19","author":[{"given":"Faiza","family":"Shaikh","sequence":"first","affiliation":[{"name":"Department of Pharmacology SVKM's Dr. Bhanuben Nanavati College of Pharmacy, Vile Parle (W)  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-9300","authenticated-orcid":false,"given":"Lokesh Kumar","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Department of Pharmacology SVKM's Dr. Bhanuben Nanavati College of Pharmacy, Vile Parle (W)  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.10.011"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1983"},{"key":"e_1_2_9_3_1","first-page":"164","article-title":"\u2018Cardiac Hypertrophy: A Review on Pathogenesis and Treatment\u2019","volume":"4","author":"Rohilla A.","year":"2012","journal-title":"Int. J. Pharm. Sci. Drug Res."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.02.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000072977.86706.23"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0922-4106(05)80032-X"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.13620"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.115.097691"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199804000-00008"},{"key":"e_1_2_9_10_1","first-page":"22","article-title":"\u2018Dantrolene reduces CaMKII\u03b4C-mediated atrial arrhythmias.\u2019","author":"Pabel S.","year":"2020","journal-title":"Europace"},{"key":"e_1_2_9_11_1","first-page":"A10562","article-title":"\u2018Abstract 10562: Dantrolene Prevents the Development of Age-Related Cardiomyopathy by Stabilization of CaMKII-Phosphorylated RyR2 in CaMKII\\&\\#x3b4;C Transgenic Mice\u2019","volume":"138","author":"Sufu-Shimizu Y.","year":"2018","journal-title":"Circulation"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00033.2006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-130"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00904"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.06.065"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1982.52.2.467"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1996.270.6.H2021"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006857213829"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.83.6.1999"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.07.006"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1215"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.04.026"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16075-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00374.2015"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.06.037"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202200309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202200309","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202200309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T06:54:15Z","timestamp":1692687255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202200309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,16]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/cbdv.202200309"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202200309","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,16]]},"assertion":[{"value":"2022-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202200309"}}