{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T03:16:33Z","timestamp":1778037393956,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T00:00:00Z","timestamp":1668124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Preparations of <jats:italic>Helicteres sacarolha<\/jats:italic> (Malvaceae) leaves and roots are used in the form of decoction, infusion or maceration, to treat gastrointestinal disturbances, among others. Studies supporting some of its ethnomedicinal uses are still incipient. The present study aimed to investigate it potential effect on chronic ulcer, ulcerative colitis and possible prokinetic activities as part of its mechanism of action. The powdered leaves of <jats:italic>Helicteres sacarolha<\/jats:italic> (HEHs) was prepared by maceration in 70\u2009% hydroethanolic solution. Its qualitative phytochemical constituents were investigated by direct flow injection analysis coupled to atmospheric pressure chemical ionization ion trap tandem mass spectrometry (FIA\u2010APCI\u2010IT\u2010MS<jats:sup>n<\/jats:sup>). The gastric ulcer healing effect was evaluated in acetic acid induced chronic ulcer in mice and the lesions were evaluated, including analysis of blood plasma cytokine levels. The prokinetic properties (gastric emptying and intestinal transit) were carried out in mice. Potential anti\u2010ulcerative colitis activity was evaluated in rats using 2,4,6\u2010trinitrobenzenesulfonic acid (5\u2009% TNBS) \u2010induced colitis. All animal experiments were carried out at the doses of 20, 50 and 250\u2005mg\/kg (p.o.). Eight compounds were putatively identified, specifically lariciresinol, and its derivatives, kaempferol derivatives and Tricin\u2010<jats:italic>O<\/jats:italic>\u2010Glc. The extract promoted increased gastric ulcer healing at all doses tested. Modulation of the cytokines involved inhibition of some key pro\u2010inflammatory cytokines with maximum effect on IL\u20101\u03b2 (70\u2009%, 50\u2005mg\/kg, <jats:italic>p<\/jats:italic>&lt;0.05), TNF\u2010\u03b1 (79\u2009%, 20\u2005mg\/kg, <jats:italic>p<\/jats:italic>&lt;0.01), and in the anti\u2010inflammatory cytokines, namely IL\u201010 (57\u2009%, 50\u2005mg\/kg, <jats:italic>p<\/jats:italic>&lt;0.05) and IL\u201017 (79\u2009%, only at 50\u2005mg\/kg, <jats:italic>p<\/jats:italic>&lt;0.05). Histological findings demonstrated a mitigated inflammatory activity, and tissues undergoing regeneration. HEHs treatment caused delayed gastric emptying, and increased intestinal transit, but had no effect in the experimentally induced ulcerative colitis. We report for the first time putatively the presence of Lariciresinol and tricin derivatives from the hydroethanolic leaves extract of <jats:italic>H. sacarolha<\/jats:italic>. Its possible mechanism of actions of gastric ulcer healing involves cytokines modulation, mitigation of inflammatory response and tissue regeneration and provoked opposing effect in the gastrointestinal system. The present study demonstrates the therapeutic potential of <jats:italic>H. sacarolha<\/jats:italic> leaves used in Brazilian ethnomedicine in the treatment of chronic gastric ulcer.<\/jats:p>","DOI":"10.1002\/cbdv.202200322","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T12:45:57Z","timestamp":1666356357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evidence for the Involvement of Cytokines Modulation and Prokinetic Properties in Gastric Ulcer Healing Effects of <i>Helicteres sacarolha<\/i> A. St.\u2010Hil. A. Juss."],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4797-6129","authenticated-orcid":false,"given":"Sikiru","family":"Olaitan Balogun","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias da Sa\u00fade Universidade Federal da Grande Dourados (UFGD)  79804-970 Mato Grosso do Sul Brazil"},{"name":"\u00c1rea de Farmacologia Departamento de Ci\u00eancias B\u00e1sicas em Sa\u00fade Faculdade de Medicina Universidade Federal de Mato Grosso (UFMT)  Cuiab\u00e1 MT Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amilcar","family":"Sabino Damazo","sequence":"additional","affiliation":[{"name":"Area of Histology Department of Basic Health Sciences Faculty of Medicine Universidade Federal de Mato Grosso (UFMT)  78060-900, MT Cuiab\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduarda","family":"Pavan","sequence":"additional","affiliation":[{"name":"\u00c1rea de Farmacologia Departamento de Ci\u00eancias B\u00e1sicas em Sa\u00fade Faculdade de Medicina Universidade Federal de Mato Grosso (UFMT)  Cuiab\u00e1 MT Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"de Freitas Figueiredo","sequence":"additional","affiliation":[{"name":"\u00c1rea de Farmacologia Departamento de Ci\u00eancias B\u00e1sicas em Sa\u00fade Faculdade de Medicina Universidade Federal de Mato Grosso (UFMT)  Cuiab\u00e1 MT Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-7068","authenticated-orcid":false,"given":"Karuppusamy","family":"Arunachalam","sequence":"additional","affiliation":[{"name":"\u00c1rea de Farmacologia Departamento de Ci\u00eancias B\u00e1sicas em Sa\u00fade Faculdade de Medicina Universidade Federal de Mato Grosso (UFMT)  Cuiab\u00e1 MT Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Sa\u00fade e Desenvolvimento da Regi\u00e3o Centro-Oeste Centro de Estudos em C\u00e9lulas Tronco Terapia Celular e Gen\u00e9tica Toxicol\u00f3gica (CeTroGen) Faculdade de Medicina Dr. H\u00e9lio Mandetta (FAMED) Universidade Federal de Mato Grosso do Sul (UFMS)  Campo Grande Mato Grosso do Sul Brazil"},{"name":"Key Laboratory of Economic Plants and Biotechnology and the Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences  Kunming 650201 P. 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