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Following quantitative HPLC analysis of the extracts, usnic acid was isolated by preparative chromatography. The study of cytotoxic activity was performed using the MTT test on three melanoma cell lines \u2013 HTB140, A375 and WM793. The selectivity of action was also assessed by comparing the effect towards normal human keratinocytes HaCaT. The results showed a dose\u2010dependent cytotoxic activity of the extracts tested and usnic acid itself, but no relationship was found between the content of usnic acid and the activity of the extracts. Furthermore, the extracts showed varied, but rather low anti\u2010tyrosinase activity. Other<jats:italic>in\u2005vitro<\/jats:italic>and<jats:italic>in\u2005vivo<\/jats:italic>studies are necessary to demonstrate that<jats:italic>C.\u2005mitis<\/jats:italic>extracts may be useful in the adjuvant external treatment of skin melanoma.<\/jats:p>","DOI":"10.1002\/cbdv.202200408","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T11:50:29Z","timestamp":1654170629000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Antimelanoma Potential of<i>Cladonia mitis<\/i>Acetone Extracts \u2013 Comparative<i>in Vitro<\/i>Studies in Relation to Usnic Acid Content"],"prefix":"10.1002","volume":"19","author":[{"given":"Marta","family":"Grudzi\u0144ska","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Jagiellonian University Medical College Medyczna 9 30-688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4821-4492","authenticated-orcid":false,"given":"Pawe\u0142","family":"Pa\u015bko","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Nutrition Faculty of Pharmacy Jagiellonian University Medical College Medyczna 9 30-688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9780-7924","authenticated-orcid":false,"given":"Dagmara","family":"Wr\u00f3bel\u2010Biedrawa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Jagiellonian University Medical College Medyczna 9 30-688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1251-7192","authenticated-orcid":false,"given":"Irma","family":"Podolak","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Jagiellonian University Medical College Medyczna 9 30-688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-8646","authenticated-orcid":false,"given":"Agnieszka","family":"Galanty","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Jagiellonian University Medical College Medyczna 9 30-688 Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"crossref","unstructured":"S.\u2005D. 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