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However, the structural property, immunomodulatory effects and antitumor activities of alcohol\u2010soluble <jats:italic>Bletilla striata<\/jats:italic> polysaccharide were rarely reported. In this study, an alcohol\u2010soluble <jats:italic>Bletilla striata<\/jats:italic> polysaccharide was firstly extracted, investigated the structural property and evaluated the antitumor activity <jats:italic>in\u2005vivo<\/jats:italic> and <jats:italic>in\u2005vitro<\/jats:italic>. Results showed that BSAP was a low molecular weight polysaccharide (2.29\u00d710<jats:sup>4<\/jats:sup>\u2005Da) and consisted of glucose, xylose and mannose (molar ratio: 2.39\u2009:\u20091.00\u2009:\u20090.21). Animal experiments results suggested that BSAP could effectively inhibit the expansion of H22 solid tumors, protect thymus and spleen, improve macrophages, lymphocytes and NK cells activities and enhance lymphocyte subsets proportion, presenting a better immunological enhancement effect <jats:italic>in\u2005vivo<\/jats:italic>. Additionally, the results of cell experiments showed that BSAP had obvious antitumor effect <jats:italic>in\u2005vitro<\/jats:italic>, including inhibiting the proliferation of H22 cells and inducing the apoptosis of tumor cells. These results would provide theoretical basis and new ideas for the further development and utilization of BSAP in the biomedical field.<\/jats:p>","DOI":"10.1002\/cbdv.202200635","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T17:53:33Z","timestamp":1666720413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Structural Characterization of an Alcohol\u2010Soluble Polysaccharide from <i>Bletilla striata<\/i> and Antitumor Activities <i>in Vivo<\/i> and <i>in Vitro<\/i>"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5287-9190","authenticated-orcid":false,"given":"Chao","family":"Liu","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Tianjin University of Science and Technology  No. 29, 13<sup>th<\/sup> Street, TEDA Tianjin 300457 P. R. 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