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A mouse model was established to mimic human IIRI by clamping the superior mesenteric artery. Transcriptomics techniques were used in conjunction with experiments to explore the potential mechanisms of EG action. Intestinal histomorphological damage, including intestinal villi damage and mucosal hemorrhage, was significantly reversed by EG. EG also alleviated the oxidative stress, inflammation, and intestinal epithelial apoptosis caused by IIRI. 2592 up\u2010regulated genes and 2754 down\u2010regulated genes were identified after EG treatment, and these differential genes were enriched in signaling pathways, including fat digestion and absorption, and extracellular matrix (ECM) receptor interactions. In IIRI mouse intestinal tissue, expression of the differential protein matrix metalloproteinase 9 (MMP9), as well as its co\u2010protein NF\u2010\u03baB\u2010p65, was significantly increased, while EG inhibited the expression of MMP9 and NF\u2010\u03baB\u2010p65. In Caco\u20102 cells in an established oxygen\u2010glucose deprivation\/reperfusion model (OGD\/R), EG significantly reversed the decrease in intestinal barrier <jats:italic>trans\u2010<\/jats:italic>epithelial electrical resistance (TEER). However, in the presence of MMP9 inhibitors, EG did not reverse the decreasing trend in TEER. This study illustrates the protective effect and mechanism of action of EG on IIRI and, combined with <jats:italic>in\u2005vivo<\/jats:italic> and <jats:italic>in\u2005vitro<\/jats:italic> experiments, it reveals that MMP9 may be the main target of EG action. This study provides new scientific information on the therapeutic effects of EG on IIRI.<\/jats:p>","DOI":"10.1002\/cbdv.202200643","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T03:25:07Z","timestamp":1670988307000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Protective Mechanism of Ethyl Gallate against Intestinal Ischemia\u2010Reperfusion Injury in Mice by in <i>Vivo<\/i> and <i>in Vitro<\/i> Studies Based on Transcriptomics"],"prefix":"10.1002","volume":"20","author":[{"given":"Shuyuan","family":"Fan","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering Liaoning Normal University  Dalian 116029 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Liaoning Normal University  Dalian 116029 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Liaoning Normal University  Dalian 116029 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dalian Medical University  Dalian 116044 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunpeng","family":"Diao","sequence":"additional","affiliation":[{"name":"College of Pharmacy Dalian Medical University  Dalian 116044 China"},{"name":"Dalian Anti-Infective Traditional Chinese Medicine, Development Engineering Technology Research Center  China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,27]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"crossref","unstructured":"L. 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