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The synthesized Schiff base was used for complexation with Cu(II) and Fe(II) ions used by a molar (2\u2009:\u20091\u2005mmol ration) in methanol solvent. The structural features of ligand, Cu(II), and Fe(II) metal complexes were determined by standard spectroscopic methods (FT\u2010IR, elemental analysis, proton and carbon NMR spectra, UV\/VIS, and mass spectroscopy, magnetic susceptibility, thermal analysis, and powder X\u2010ray diffraction). The synthesized compounds (Schiff base and its metal complexes) were screened in terms of their anti\u2010proliferative activities in U118 and T98G human glioblastoma cell lines alone or in combination with electroporation (EP). Moreover, the human HDF (human dermal fibroblast) cell lines was used to check the bio\u2010compatibility of the compounds. Anti\u2010proliferative activities of all compounds were ascertained using an MTT assay. The complexes exhibited a good anti\u2010proliferative effect on U118 and T98G glioblastoma cell lines. In addition, these compounds had a negligible cytotoxic effect on the fibroblast HDF cell lines. The use of compounds in combination with EP significantly decreased the IC<jats:sub>50<\/jats:sub> values compared to the use of compounds alone (p&lt;0.05). These results show that newly synthesized Cu(II) and Fe(II) complexes can be developed for use in the treatment of chemotherapy\u2010resistant U118 and T98G glioblastoma cells and that treatment with lower doses can be provided when used in combination with EP.<\/jats:p>","DOI":"10.1002\/cbdv.202200710","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T10:05:10Z","timestamp":1672913110000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Electroporation Enhances the Anticancer Effects of Novel Cu(II) and Fe(II) Complexes in Chemotherapy\u2010Resistant Glioblastoma Cancer Cells"],"prefix":"10.1002","volume":"20","author":[{"given":"Mehmet Esref","family":"Alkis","sequence":"first","affiliation":[{"name":"Department of Occupational Health and Safety Faculty of Health Sciences Mu\u015f Alparslan University  49250 Mu\u015f 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