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Legrand (Myrtaceae) displays antiulcer properties when given to rodents. The major active chemical components of <jats:italic>C.\u2005reitziana<\/jats:italic> are chalcones, including 4\u2032,6\u2032\u2010dihydroxy\u20102\u2032\u2010methoxy\u20103\u2032,5\u2032\u2010dimethylchalcone or dimethyl cardamonin (DMC); therefore, we hypothesized that this compound could have antiulcer effects and the present study aimed to evaluate its gastroprotective and gastric healing properties. DMC was isolated from the fruits of <jats:italic>C.\u2005reitziana<\/jats:italic>, and its gastroprotective effect was evaluated by ethanol and indomethacin\u2010induced gastric ulcer models in mice (0.1\u2005mg\/kg, i.p. and 1 and 3\u2005mg\/kg, p.o.). Oxidative stress and inflammatory parameters were analyzed in the gastric tissue. Moreover, its gastric healing effect was evaluated in rats. In addition, the compound's mode of action was evaluated <jats:italic>in\u2005vivo<\/jats:italic> and <jats:italic>in\u2005vitro<\/jats:italic> by measuring H<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010ATPase activity. Finally, the cytotoxic potential of DMC was tested in fibroblasts and human gastric adenocarcinoma cells. The DMC reduced the ethanol\u2010induced gastric ulcer in mice by 77\u2009%, increased the adhered mucus, and reduced lipoperoxides levels. The block of nonprotein sulfhydryls (NP\u2010SH) compounds by pretreatment with N\u2010ethylmaleimide (NEM), the inhibition of nitric oxide synthase with N\u03c9\u2010nitro\u2010L\u2010arginine methyl ester hydrochloride (L\u2010NAME), or the antagonism of \u03b1<jats:sub>2<\/jats:sub> receptor using yohimbine reversed the gastroprotective effects of DMC. Furthermore, DMC reduced the acidity of gastric content in pylorus\u2010ligated rats but did not change H<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>\u2010ATPase (isolated from rabbit) activity <jats:italic>in\u2005vitro<\/jats:italic>. DMC reduced the lesion area in acetic acid\u2010induced ulcers and decreased myeloperoxidase activity. DMC did not change the viability of fibroblast cells (L929) but reduced the viability of human gastric adenocarcinoma cells (AGS). The results confirmed that DMC could significantly enhance the gastric healing process and prevent ulcers due to improving protective factors on the gastric mucosa and reducing gastric acid secretion.<\/jats:p>","DOI":"10.1002\/cbdv.202200727","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T15:02:10Z","timestamp":1666018930000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dimethyl Cardamonin from Fruits of <i>Campomanesia reitziana<\/i> D. Legrand Promotes Gastroprotection and Gastric Healing Effects in Rodents"],"prefix":"10.1002","volume":"19","author":[{"given":"Benhur Judah","family":"Cury","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas N\u00facleo de Investiga\u00e7\u00f5es Qu\u00edmico-Farmac\u00eauticas Universidade do Vale do Itaja\u00ed  Rua Uruguai, 458, Centro 88302\u2013901 Itaja\u00ed Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaise","family":"Boeing","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas N\u00facleo de Investiga\u00e7\u00f5es Qu\u00edmico-Farmac\u00eauticas Universidade do Vale do Itaja\u00ed  Rua Uruguai, 458, Centro 88302\u2013901 Itaja\u00ed Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lincon 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Investiga\u00e7\u00f5es Qu\u00edmico-Farmac\u00eauticas Universidade do Vale do Itaja\u00ed  Rua Uruguai, 458, Centro 88302\u2013901 Itaja\u00ed Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"de Souza","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas N\u00facleo de Investiga\u00e7\u00f5es Qu\u00edmico-Farmac\u00eauticas Universidade do Vale do Itaja\u00ed  Rua Uruguai, 458, Centro 88302\u2013901 Itaja\u00ed Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa Mota","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas N\u00facleo de Investiga\u00e7\u00f5es Qu\u00edmico-Farmac\u00eauticas Universidade do Vale do Itaja\u00ed  Rua Uruguai, 458, Centro 88302\u2013901 Itaja\u00ed Santa Catarina 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